GeneQuery™ 人源免疫应答凝集素调控相关基因 qPCR 阵列检测试剂盒
本试剂盒可系统分析 40 个在病原体特异性免疫应答中发挥作用的凝集素受体基因的表达情况。
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This Item:
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First-Strand cDNA Synthesis Master Mix, 4x
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GoldNStart TaqGreen qPCR Master Mix
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ScienCell's GeneQuery™ Human Lectin Regulation in Immune Response qPCR Array Kit (GQH-LRI) is designed to facilitate gene expression profiling of 40 selected lectin receptors that are involved in pathogen-specific immune responses. Brief examples of how included genes may be grouped according to their functions are shown below:
- C-Type Lectin Receptors: ASGR1, CD69, CD207, CD302, CLEC3B, CLEC4A-M, CLEC5A, CLEC6A, CLEC7A, CLEC10A, CLEC16A, LY75, MRC1, MRC2
- NK Cell lectin-like receptors: KLRB1, KLRC1, KLRC2, KLRD1, KLRK1
- Other subgroups such as selectins, chondrolectins and collectins: ACAN, CD72, CD93, CD248, MBL2, OLR1, PKD1, SELE, SELL, SELP, SFTPA2, SFTPD, THBD
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.
ScienCell 的 GeneQuery™ 人类免疫应答中凝集素调控相关基因 qPCR 数组试剂盒(GQH-LRI) 可用于分析 40 个参与病原体特异性免疫反应的凝集素受体基因的表达谱。
试剂盒中所涵盖的基因可根据功能大致分类如下:
- C 型凝集素受体(C-Type Lectin Receptors): ASGR1、CD69、CD207、CD302、CLEC3B、CLEC4A-M、CLEC5A、CLEC6A、CLEC7A、CLEC10A、CLEC16A、LY75、MRC1、MRC2
- NK 细胞类凝集素受体(NK Cell lectin-like receptors): KLRB1、KLRC1、KLRC2、KLRD1、KLRK1
- 其他亚类(如 selectins、chondrolectins 和 collectins): ACAN、CD72、CD93、CD248、MBL2、OLR1、PKD1、SELE、SELL、SELP、SFTPA2、SFTPD、THBD
GeneQuery™ qPCR 数组试剂盒采用 96 孔板即用型(qPCR-ready)设计,每个孔中预装一套特异性引物,可高效识别并扩增对应目标基因的 cDNA。
精心设计的引物确保:
- 在标准 qPCR 条件下具有最佳退火温度 65°C(反应体系含 2 mM Mg²⁺,不含 DMSO);
- 除非另有说明,引物可识别目标基因的所有已知转录变体;
- 每套引物仅扩增单一目标基因,确保扩增特异性。
所有引物均已通过 qPCR 熔解曲线分析及凝胶电泳验证,以保证实验结果的准确性和可靠性。
| 目录编号 | GK111 |
|---|---|
| 制造国家 | 美国 |
| 产品编码 | GQH-LRI |
| 规格/数量 | One 96-well plate, one 20ul reaction per well |
| 产品用途 | 本产品仅供研究使用。未经批准,不得用于人体、动物或体外诊断程序。 |
| 储存 | 收到后请保存在4℃。如需长期保存(超过1年),请将板材保存在-20℃的手动除霜冰箱中。 |
| 运输 | 常温。 |
