血管平滑肌细胞（SMC）是动脉疾病发生发展的主要贡献者。血管SMC转变为增殖表型的能力是血管疾病发生和进展的主要因素之一。血管平滑肌细胞表达ICAM1和VCAM-1，这些分子可能促进血管壁中的炎症反应，并可能积极参与血管疾病的进展。体外培养的血管SMC在血管疾病研究中发挥重要作用，并可用于鉴定治疗动脉疾病的新靶点。
ScienCell Research Laboratories提供的HUASMC来源于人脐动脉。HUASMC在第1代传代时冷冻保存并以冻存状态提供。每瓶含有 >5 x 105 个细胞，体积为1 ml。HUASMC通过特异性识别α-平滑肌肌动蛋白的抗体进行免疫荧光鉴定。HUASMC检测结果为HIV-1、HBV、HCV、支原体、细菌、酵母和真菌阴性。在ScienCell Research Laboratories提供的条件下，HUASMC保证可进一步扩增15次群体倍增。
推荐培养基
建议在体外培养HUASMC时使用平滑肌细胞培养基（SMCM，目录号#1101）。
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The construction of a smooth muscle layer for blood vessel through electrospinning method plays a key role in vascular tissue engineering. However, smooth muscle cells (S... More
The construction of a smooth muscle layer for blood vessel through electrospinning method plays a key role in vascular tissue engineering. However, smooth muscle cells (SMCs) penetration into the electrospun graft to form a smooth muscle layer is limited due to the dense packing of fibers and lack of inducing factors. In this paper, silk fibroin/poly (L-lactide-ε-caplacton) (SF/PLLA-CL) vascular graft loaded with platelet-rich growth factor (PRGF) was fabricated by electrospinning. The in vitro results showed that SMCs cultured in the graft grew fast, and the incorporation of PRGF could induce deeper SMCs infiltrating compared to the SF/PLLA-CL graft alone. Mechanical properties measurement showed that PRGF-incorporated graft had proper tensile stress, suture retention strength, burst pressure and compliance which could match the demand of native blood vessel. The success in the fabrication of PRGF-incorporated SF/PLLA-CL graft to induce fast SMCs growth and their strong penetration into graft has important application for tissue-engineered blood vessels. Less
Objective ATP-binding cassette transporters(ABC) Al and Gl play an important role in mediating cholesterol efflux and preventing macrophage foam cell formation. In this s... More
Objective ATP-binding cassette transporters(ABC) Al and Gl play an important role in mediating cholesterol efflux and preventing macrophage foam cell formation. In this study, we examined the regulation of ABC transporters by high glucose in human vascular smooth muscle cells(VSMCs), the other precursor of foam cells. Methods Incubation of human VSMCs with D-glucose(5 to 30 mM) for 1 to 7 days in the presence or absence of antioxidant and nuclear factor(NF)- κ B inhibitors, the expressions of ABCA1 and ABCG1 were analyzed by real time PCR and Western blotting. Results High glucose decreased ABCG1 mRNA and protein expression in cultured VSMCs, whereas the expression of ABCA1 was not significantly decreased. Down-regulation of ABCG1 mRNA expression by high glucose was abolished by antioxidant N-acetyl-L-cysteine(NAC) and NF- κ B inhibitors, BAY 11-7085 and tosyl-phenylalanine chloromethyl-ketone(TPCK). Conclusion High glucose suppresses the expression of ABCG1 in VSMCs, which is the possible mechanism of VSMC derived foam cell transformation. Less
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