牛源星形胶质细胞

Astrocytes are the most abundant cell type in the central nervous system (CNS) and they provide a variety of vital functions for the CNS including: establishment and regulation of the blood brain barrier, functional support for neuronal transmission, survival of neurons, anti-inflammatory responses and wound repair. Astrocytes have also been implicated in various pathological processes such as reactive gliosis. Impairment of normal astrocyte functions during stroke and other insults can critically influence neuron survival. Long-term recovery after brain injury, through neurite outgrowth, synaptic plasticity, or neuron regeneration, is also influenced by astrocyte surface molecule expression and trophic factor release. Numerous studies have demonstrated that astrocytes are among the most functionally diverse group of cells in the CNS. Bovine astrocytes (BA) are a useful in vitro model for studying glial function and the molecular mechanisms of CNS diseases such as ischemic stroke and multiple sclerosis.

BA from ScienCell Research Laboratories are isolated from bovine brain. BA are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. BA are characterized by immunofluorescence with antibody specific to GFAP. BA are negative for mycoplasma, bacteria, yeast, and fungi. BA are guaranteed to further expand for 5 population doublings under the conditions provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Astrocyte Medium-animal (AM-a, Cat. #1831) for culturing BA in vitro.

星形胶质细胞是中枢神经系统（CNS）中数量最多的细胞类型之一，在维持中枢神经系统功能方面发挥多种关键作用，包括血脑屏障的建立与调控、神经传递的功能支持、神经元存活维持、抗炎反应以及损伤修复等。星形胶质细胞还与多种病理过程相关，例如反应性胶质增生。在脑卒中及其他损伤情况下，星形胶质细胞正常功能的受损会显著影响神经元的存活。脑损伤后的长期恢复过程，例如神经突生长、突触可塑性以及神经元再生，也受到星形胶质细胞表面分子表达及营养因子释放的影响。大量研究表明，星形胶质细胞是中枢神经系统中功能最为多样化的细胞群之一。牛源星形胶质细胞（BA）是研究胶质细胞功能以及中枢神经系统疾病（如缺血性卒中和多发性硬化症）分子机制的重要体外模型。

ScienCell Research Laboratories 的牛源星形胶质细胞（BA）分离自牛脑组织。BA 于第一代（Passage 1）进行冻存，并以冷冻状态运输。每瓶含有超过 5 × 10⁵ 个细胞，体积为 1 ml。BA 通过针对 GFAP 的特异性抗体进行免疫荧光鉴定。经检测，BA 不含支原体、细菌、酵母及真菌污染。在 ScienCell Research Laboratories 提供的培养条件下，BA 可保证继续扩增 5 次群体倍增。

推荐培养基

建议使用 Astrocyte Medium-animal（AM-a，产品编号：1831）进行 BA 的 in vitro 培养。