兔源星形胶质细胞

Astrocytes are the most abundant cell type in the central nervous system (CNS) and they provide a variety of vital functions for the CNS including: establishment and regulation of the blood brain barrier, functional support for neuronal transmission, survival of neurons, anti-inflammatory responses and wound repair. Astrocytes have also been implicated in various pathological processes such as reactive gliosis. Impairment of normal astrocyte functions during stroke and other insults can critically influence neuron survival. Long-term recovery after brain injury, through neurite outgrowth, synaptic plasticity, or neuron regeneration, is also influenced by astrocyte surface molecule expression and trophic factor release. Numerous studies have demonstrated that astrocytes are among the most functionally diverse group of cells in the CNS. Rabbit astrocytes (RabA) are a useful in vitro model for studying glial function and the molecular mechanisms of CNS diseases such as ischemic stroke and multiple sclerosis.

RabA from ScienCell Research Laboratories are isolated from rabbit brain. RabA are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. RabA are characterized by immunofluorescence with antibody specific to GFAP. RabA are negative for mycoplasma, bacteria, yeast, and fungi. RabA are guaranteed to further expand for 10 population doublings under the conditions provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Astrocyte Medium-animal (AM-a, Cat. #1831) for culturing RabA in vitro.

星形胶质细胞是中枢神经系统（CNS）中最丰富的细胞类型，在CNS中发挥多种关键功能，包括血脑屏障的建立与调控、对神经元信号传递的功能支持、促进神经元存活、介导抗炎反应以及参与损伤修复等。星形胶质细胞还参与多种病理过程，例如反应性胶质增生。在脑卒中及其他损伤情况下，星形胶质细胞正常功能的受损会对神经元的存活产生重要影响。脑损伤后的长期恢复过程（如神经突起生长、突触可塑性或神经再生）同样受到星形胶质细胞表面分子表达及其分泌的营养因子的调控。大量研究表明，星形胶质细胞是CNS中功能多样性最为显著的细胞类型之一。兔星形胶质细胞（RabA）是研究胶质细胞功能及中枢神经系统疾病（如缺血性脑卒中和多发性硬化）分子机制的理想体外模型。

ScienCell Research Laboratories 提供的 RabA 分离自兔脑组织。细胞在第一代（P1）时进行冷冻保存，并以冻存形式提供。每瓶含有 >5 × 10⁵ 个细胞，体积为 1 ml。RabA 通过针对 GFAP 的特异性抗体进行免疫荧光鉴定。经检测，RabA 不含支原体、细菌、酵母及真菌污染。在 ScienCell Research Laboratories 提供的培养条件下，RabA 保证可进一步扩增至 10 代倍增。

推荐培养基

建议使用 Astrocyte Medium-animal（AM-a，产品编号 #1831）进行 RabA 的 体外（in vitro） 培养。