HPSC-derived Cardiomyocytes
H9-CM are cryopreserved at passage one after enrichment and delivered frozen. Each vial contains >1 x 106 cells in 1 ml volume.
Includes one vial of H9-CM, 50mL of Cat# 5921 Cardiomyocyte Plating Medium-basal, and 1 mL of Cat# 5972 Cardiomyocyte Plating Medium Supplement (50X).
This item will be available to ship approximately 3 weeks after the order is submitted
Human primary cardiomyocytes and cardiac tissue are superior modeling systems for heart disease studies, drug discovery and toxicity testing compared to animal cells or immortalized cell lines. Human adult primary cardiomyocytes are difficult to obtain due to limited availability of cardiac tissue and they do not typically proliferate in culture. Cardiomyocytes derived from human pluripotent stem cells (HPSC) can potentially provide an unlimited supply of cardiomyocytes for drug screening, toxicity testing and developmental studies.
HPSC-derived Cardiomyocytes (H9-CM) from ScienCell Research Laboratories are differentiated from a pluripotent stem cell line (H9) and enriched for contracting cells. Through the temporal modulation of canonical Wnt signaling and growth factor induction, monolayer-cultured HPSCs are differentiated to contracting cardiomyocytes under serum-free and feeder-free conditions at high efficiency. H9-CM are cryopreserved at passage one after enrichment and delivered frozen. Each vial contains >1 x 106 cells in 1 ml volume. Cells are characterized by immunofluorescence with antibodies specific to α-Actinin and Sarcomeric Tropomyosin. H9-CM are negative for mycoplasma, bacteria, yeast and fungi. H9-CM are guaranteed to further culture under the conditions provided by ScienCell Research Laboratories. H9-CM are not recommended for expanding since the cells show limited proliferation capability in culture.
Recommended Medium
It is recommended to use Cardiac Myocyte Medium (CMM, Cat. #6201) for the culturing of H9-CM in vitro.
| Catalog No. | 6250 |
|---|---|
| Country of Manufacture | United States |
| Product Code | H9-CM |
| Size/Quantity | 1 x 10^6 cells/vial |
| Product use | This product is for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures. |
| Storage | Upon receiving, directly and immediately transfer Cat. #6250 from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments. Store Cat. #5921 at 4°C in the dark and Cat. #5972 at -20°C. |
| Shipping | Cat. #6250 and Cat. #5972 are shipped on dry ice. Cat. #5921 is shipped at room temperature. |
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