Rat Mesenchymal Stem Cells-bone marrow (RMSC-bm) - phase contrast 100x

Rat Mesenchymal Stem Cells-bone marrow

Name: Rat Mesenchymal Stem Cells-bone marrow
Brand: Sciencell Research Laboratories
SKU: R7500
Price: 471 USD
Availability: InStock

In Stock

Catalog No.

R7500

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$471.00

Rat Mesenchymal Stem Cells-bone marrow (RMSC-bm) isolated from adult rat bone marrow. RMSC-bm are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume.

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Mesenchymal stem cells (MSC) are a well-characterized population of adult stem cells. They have the potential to develop into mature cells that produce fat, cartilage, bone, tendons, and muscle. The developmental plasticity of MSC has generated tremendous interest because of the potential use of mesenchymal stem cells in regenerative medicine to replace damaged tissues. MSC cultured without serum in the presence of transformation growth factors will differentiate into chondrocytes. In contrast, MSC cultured in serum with ascorbic acid and dexamethasone will differentiate into osteoblasts. With their renewal capability, MSC have the potential to be transplanted into an injured site or seeded on a biomimetic scaffold to generate appropriate tissue constructs.

RMSC-bm from ScienCell Research Laboratories are isolated from adult CD® IGS rat bone marrow. RMSC-bm are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. RMSC-bm are characterized by immunofluorescence with antibodies to CD73 and/or CD90, Oil Red O staining after adipogenic differentiation, and/or Alizarin Red staining after osteogenic differentiation. RMSC-bm are negative for mycoplasma, bacteria, yeast, and fungi. RMSC-bm are guaranteed to further culture under the conditions provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Mesenchymal Stem Cell Medium (MSCM, Cat. #7501) for culturing RMSC-bm in vitro.

More Information
Catalog No.	R7500
Country of Manufacture	United States
Product Code	RMSC-bm
Size/Quantity	5 x 10^5 cells/vial
Product use	This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures.
Storage	Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture is needed for experiments.
Shipping	Dry ice.

1. Ma, Z.G., Lv, X.D., Zhan, L.L., Chen, L., Zou, Q.Y., Xiang, J.Q., Qin, J.L., Zhang, W.W., Zeng, Z.J., Jin, H., Jiang, H.X. & Lv, X.P. (2016) Human urokinase-type plasminogen activator gene-modified bone marrow-derived mesenchymal stem cells attenuate liver fibrosis in rats by down-regulating the Wnt signaling pathway World J Gastroenterol. 22

AIM: To evaluate the therapeutic effects of bone marrow-derived mesenchymal stem cells (BMSCs) with human urokinase-type plasminogen activator (uPA) on liver fibrosis, an... More

AIM: To evaluate the therapeutic effects of bone marrow-derived mesenchymal stem cells (BMSCs) with human urokinase-type plasminogen activator (uPA) on liver fibrosis, and to investigate the mechanism of gene therapy. METHODS: BMSCs transfected with adenovirus-mediated human urokinase plasminogen activator (Ad-uPA) were transplanted into rats with CCl4-induced liver fibrosis. All rats were sacrificed after 8 wk, and their serum and liver tissue were collected for biochemical, histopathologic, and molecular analyzes. The degree of liver fibrosis was assessed by hematoxylin and eosin or Masson’s staining. Western blot and quantitative reverse transcription-polymerase chain reaction were used to determine protein and mRNA expression levels. RESULTS: Serum levels of alanine aminotransferase, aminotransferase, total bilirubin, hyaluronic acid, laminin, and procollagen type III were markedly decreased, whereas the levels of serum albumin were increased by uPA gene modified BMSCs treatment. Histopathology revealed that chronic CCl4-treatment resulted in significant fibrosis while uPA gene modified BMSCs treatment significantly reversed fibrosis. By quantitatively analysing the fibrosis area of liver tissue using Masson staining in different groups of animals, we found that model animals with CCl4-induced liver fibrosis had the largest fibrotic area (16.69% ± 1.30%), while fibrotic area was significantly decreased by BMSCs treatment (12.38% ± 2.27%) and was further reduced by uPA-BMSCs treatment (8.31% ± 1.21%). Both protein and mRNA expression of β-catenin, Wnt4 and Wnt5a was down-regulated in liver tissues following uPA gene modified BMSCs treatment when compared with the model animals. CONCLUSION: Transplantation of uPA gene modified BMSCs suppressed liver fibrosis and ameliorated liver function and may be a new approach to treating liver fibrosis. Furthermore, treatment with uPA gene modified BMSCs also resulted in a decrease in expression of molecules of the Wnt signaling pathway. Less

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