Iron Assay
The Iron Assay kit measures the total iron level of different types of samples, such as cell lysate, tissue extracts, serum and plasma. This product is for research purposes only and not for use in animals, humans, or diagnostic procedures.
Iron is an element that plays an essential role in a number of biological processes. Iron is bound to several proteins such as the oxygen-transporting hemoglobin in the red blood cells and oxygen storage protein myoglobin in the muscle. It is also stored by ferritin and hemosiderin. Inorganic iron contributes to redox reactions in the iron-sulfur clusters of many enzymes, such as nitrogenase. In this colorimetric assay, ferric carrier protein will dissociate ferric into solution under acidic conditions. After they are reduced to the ferrous ions, these ions react with chromogen to produce a stable colored complex and give absorbance at 590 nm. The color intensity thus formed is directly proportional to the concentration of iron in the sample. A specific chelate chemical is included in the buffer to block copper ion interference.
| Catalog No. | 8448 |
|---|---|
| Country of Manufacture | United States |
| Product Code | IR |
| Size/Quantity | 100 Tests |
| Product use | This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. |
| Storage | Store in -20°C |
| Shipping | Dry ice. |
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