Human uPA/PLAU ELISA Kit

The urokinase-type plasminogen activator (uPA, PLAU) converts plasminogen to plasmin. Plasmin is involved in the processing of amyloid precursor protein and degrades secreted and aggregated amyloid-β, a hallmark of Alzheimer's disease (AD). Urokinase has a molecular mass of about 54 kDa and is composed of 2 disulfide-linked chains, A and B, which have molecular masses 18 kDa and 33 kDa, respectively. uPA localizes to chromosome 10q24. uPA facilitates cell migration by localizing proteolysis on the cell surface and by inducing intracellular signaling pathways. In human vascular smooth muscle cells (VSMC), uPA stimulates migration via the uPA receptor (uPAR) signaling complex containing TYK2 and phosphatidylinositol 3-kinase (PI3-K).

ScienCell's human uPA ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. Human uPA-specific polyclonal antibodies are pre-coated onto 8 x 12 strips. The human-specific detection polyclonal antibodies are biotinylated. The test samples and biotinylated detection antibodies are subsequently added to the wells and then washed with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes to yellow after adding acidic stop solution. The intensity of yellow is proportional to the amount of human uPA in the sample that is captured on the strips.