GeneQuery™ Human Mesenchymal Stem Cell Adipogenesis qPCR Array Kit
ScienCell's GeneQuery™ Human Mesenchymal Stem Cell Adipogenesis qPCR Array Kit (GQH-MAD) is designed to facilitate gene expression profiling of 88 key genes involved in mesenchymal stem cell (MSC) adipogenic lineage commitment and differentiation. MSCs have the capacity to self-renew while maintaining stemness, and have the potential to differentiate into specialized cell lineages including adipocytes. The PPAR-gamma signaling pathway plays a key role in the induction and regulation of the adipogenesis process
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ScienCell's GeneQuery™ Human Mesenchymal Stem Cell Adipogenesis qPCR Array Kit (GQH-MAD) is designed to facilitate gene expression profiling of 88 key genes involved in mesenchymal stem cell (MSC) adipogenic lineage commitment and differentiation. MSCs have the capacity to self-renew while maintaining stemness, and have the potential to differentiate into specialized cell lineages including adipocytes. The PPAR-gamma signaling pathway plays a key role in the induction and regulation of the adipogenesis process. Brief examples of how genes may be grouped are shown below:
- MSC markers: ALCAM, ANPEP, CASP3, CD44, ENG, ERBB2, ITGA1/6/V, MCAM, NGFR, NT5E, PDGFRA/B, THY1, VCAM1, VIM
- Stemness markers: FGF2, FUT4, INS, LIF, POU5F1, PROM1, SOX2, TERT, WNT3A, ZFP42
- Adipogenic commitment regulation: ADIPOR1/2, AXIN1, CCND1, CEBPB/D, CIDEA, DLK1/2, EGR2, FGF1, FOXO1, GATA2/3, KLFs, LEP, LEPR, RHOA, SLC27A1, TNFRSF9, VDR
- PPAR-gamma signaling: ADIPOQ, AGT, CEBPA, CREB1, FABP4, IRS2, KLF15, PPARG, RETN, SIRT3, SREBF1, STAT5A
- Fully differentiated adipocyte markers: FASN, LIPE, LPL, PPARGC1A, SLC2A4, UCP1
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.
| Catalog No. | GK129 |
|---|---|
| Country of Manufacture | United States |
| Product Code | GQH-MAD |
| Size/Quantity | One 96-well plate, one 20ul reaction per well. |
| Product use | This product is for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures. |
| Storage | Upon receipt, the plate should be stored at 4°C and is good for up to 12 months. For long-term storage (>1 year), store the plate at -20°C in a manual defrost freezer. |
| Shipping | Ambient temperature. |
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