Collagenase Activity Assay

Collagenase is an enzyme in the matrix metalloproteinase family that breaks down collagen. This assists in the degradation of the extracellular matrix and tissue remodeling, which is a key step in the pathogenesis of bacteria. In the ScienCell Collagenase Activity Assay, collagenase activity is measured using a synthetic peptide substrate, FALGPA, which mimics the structure of collagen. This assay is suitable for bacterial collagenases, such as those from Clostridium histolyticum, and for screening collagenase inhibitors. The peptide is cleaved by collagenase and the resulting activity can be quantified using a microplate reader at an absorbance of 345 nm in kinetic mode. The kit offers a detection limit of 0.02 mU and provides results within 5–15 minutes.