3D-Mammary Fibroblast Spheroid Medium
3D-Mammary Fibroblast Spheroid Medium (3D-HMFSpM) is a specialized medium designed for the generation and maintenance of spheroids consisting of human mammary fibroblasts in vitro in conjunction with fetal bovine serum (FBS, Cat. #0010) and 3D-mammary fibroblast spheroid supplement (3D-HMFSpS, Cat. #3D-7632). 3D-HMFSpM is a sterile, viscous medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, and trace minerals. The medium is bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air.
3D-HMFSpM consists of 200 mL of basal medium, 10 mL of FBS (Cat. #0010), 2 mL of 3D-mammary fibroblast spheroid supplement (3D-HMFSpS, Cat. #3D-7632), and 2 mL penicillin/streptomycin solution (P/S, Cat. #0583). Note: 3D-HMFSpS, FBS, and P/S are not pre-mixed in the 3D-HMFSpM; they must be added separately to make the complete medium.
| Catalog No. | 3D-7631 |
|---|---|
| Country of Manufacture | United States |
| Product Code | 3D-HMFSpM |
| Size/Quantity | 200 mL of basal medium, 10 mL of FBS, 2 mL of 3D-HMFSpS, and 2 mL of P/S |
| Product use | 3D-HMFSpM is for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures. |
| Storage | Store the basal medium at 4oC and the 3D-HMFSpS, FBS, and P/S solution at -20°C. Protect from light. |
| Shipping | Basal medium: room temperature. Supplements: dry ice. |
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