All-Inclusive 3D Human Hepatic Stellate-Endothelial Cell Spheroid Formation Kit
ScienCell has developed the all-inclusive 3D human hepatic stellate-endothelial cell spheroid formation kit (3D-HHSteECSF) comprised of human hepatic stellate cells and sinusoidal endothelial cells. This kit is designed for studying the cellular crosstalk between hepatic stellate cells and sinusoidal endothelial cells during liver diseases such as liver fibrosis and caner metastasis.
Liver fibrogenesis and angiogenesis are closely linked processes. For example, in physiological conditions, hepatic sinusoidal endothelial cells (HSEC) maintain hepatic stellate cell (HSteC) quiescence, thereby inhibiting intrahepatic vasoconstriction and fibrosis development. When capillarized, however, hepatic sinusoidal endothelial cells lose their capacity to inactivate hepatic stellate cells, thus promoting fibrogenesis and intrahepatic vasoconstriction. To investigate the signaling crosstalk between these cellular events, ScienCell has developed the all-inclusive 3D human hepatic stellate-endothelial cell spheroid formation kit (3D-HHSteECSF) comprised of human hepatic stellate cells and sinusoidal endothelial cells. Furthermore, immunostaining of the co-culture spheroids reveals the presence of vimentin-positive stellate cells and von willebrand factor (VWF)-positive sinusoidal endothelial cells. The spheroids also contain the activated stellate cell population marked by the smooth muscle actin (SMA) staining.
Kit Components:
| 3D Cell Culture Components | ||||
|---|---|---|---|---|
| Cat # | # of vials | Product Name | Quantity | Storage |
| 5000 | 1 | Human Hepatic Sinusoidal Endotdelial Cells (HHSEC) | 5 x 10^5 cells | Liquid nitrogen |
| 5300 | 1 | Human Hepatic Stellate Cells (HHSteC) | 5 x 10^5 cells | Liquid nitrogen |
| 3D-5201 | 1 | 3D-Hepatic Spheroid Medium (3D-HSpM) | 200 mL | 2-8 °C |
| 3D-5452 | 1 | 3D-Hepatic Stellate-Endothelial Spheroid Supplement (3D-HSteESpS) | 4 mL | -20 °C |
| 0583 | 1 | Penicillin/Streptomycin Solution (P/S) | 2 mL | -20 °C |
| 0343 (or) 0353 (or) 0383 | 2 | Ultra-Low Binding Culture Plates (24-, 48-, or 96- well plate) | 2 plates | RT |
| 2D Cell Culture Components | ||||
| Cat # | # of vials | Product Name | Quantity | Storage |
| 8711 | 1 | 2D-Hepatic Stellate-Endothelial Cell Co-culture Medium (2D-HSteECM) | 500 mL | 2-8 °C |
| 8762 | 1 | 2D-Hepatic Stellate-Endothelial Cell Co-culture Growth Supplement (2D-HSteECGS) | 5 mL | -20 °C |
| 0025 | 1 | Fetal Bovine Serum (FBS) | 25 mL | -20 °C |
| 0503 | 1 | Penicillin/streptomycin Solution (P/S) | 5 mL | -20 °C |
| Catalog No. | 3D-5000 |
|---|---|
| Country of Manufacture | United States |
| Product Code | 3D-HHSteECSF |
| Size/Quantity | 1 kit |
| Product use | 3D-HHSteECSF are for research use only. It is not approved for human or animal use, or application in clinical or in vitro diagnostic procedures. |
| Storage | Refer to product sheet. |
| Shipping | 5000, 5300, 3D-5452, 0583, 8762, 0025, and 0503 are shipped on dry ice. 3D-5201, 8711, and (0343 or 0353 or 0383) are shipped at room temperature. |
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