端粒酶活性定量 qPCR 检测试剂盒

Telomeres are repetitive nucleotide elements at the ends of chromosomes that protect chromosomes from degradation and genetic information loss. Telomerase is an RNA-containing enzyme that synthesizes the telomere repeat sequence at the 3' end of telomeres. Normal diploid cells lack telomerase activity; therefore, telomeres are lost with each cell cycle. In certain cells such as embryonic stem cells and most cancer cells, however, telomerase is activated to maintain telomere length and cellular immortality. The quantitative detection of telomerase activity is important in many aspects of cell biology, such as stemness and oncogenesis.

ScienCell's Telomerase Activity Quantification qPCR Assay Kit (TAQ) is designed to quantitatively compare telomerase activity among cell populations. The cell lysis buffer is a mild lysis buffer that enables the release of telomerase in the native state. The telomere primer set (TPS) recognizes and amplifies newly synthesized telomere sequences in the assay. The carefully designed primers ensure at suggested assay conditions: (i) high efficiency for trustworthy quantification; and (ii) no non-specific amplification. The primer set has been validated by qPCR with melt curve analysis and gel electrophoresis for amplification specificity and by template serial dilution for amplification efficiency. The 2X GoldNStart TaqGreen qPCR Master Mix (Cat #MB6018a-1) is a SYBR®Green dye-based qPCR master mix with a “hot-start” property. It contains SYBR®Green, dNTPs, Taq DNA polymerase, and an inert gold-color loading indicator in a single tube. The “hot-start” property achieved through ScienCell’s unique chemically modified Taq DNA polymerase provides maximal inhibition of primer dimer formation. The advanced buffer formulation provides superior specificity and efficiency with a wide linear dynamic range. The inert gold-color loading indicator allows for better visualization and tracking of sample
loading in qPCR plates or tubes.

端粒（Telomeres）是位于染色体末端的重复核苷酸序列，可保护染色体免于降解并防止遗传信息丢失。端粒酶是一种含 RNA 的酶，可在端粒的 3' 末端合成端粒重复序列。正常二倍体细胞缺乏端粒酶活性，因此端粒会随每一次细胞分裂逐渐缩短。然而，在某些细胞中，如胚胎干细胞及大多数癌细胞，端粒酶被激活以维持端粒长度和细胞的“永生”。对端粒酶活性进行定量检测在干细胞研究和肿瘤发生等细胞生物学研究中具有重要意义。

ScienCell 的端粒酶活性定量 qPCR 检测试剂盒（Telomerase Activity Quantification qPCR Assay Kit，TAQ）可用于在不同细胞群体之间定量比较端粒酶活性。试剂盒中的细胞裂解缓冲液为温和型，可在保留端粒酶天然状态的前提下释放酶活性。端粒引物组（TPS）可识别并扩增实验中新合成的端粒序列。精心设计的引物确保在推荐实验条件下：(i) 高扩增效率，实现可靠定量；(ii) 无非特异性扩增。引物组已通过 qPCR 熔解曲线分析和凝胶电泳验证其扩增特异性，并通过模板梯度稀释实验验证其扩增效率。

试剂盒中包含 2X GoldNStart TaqGreen qPCR Master Mix（Cat #MB6018a-1），这是一种基于 SYBR® Green 的 qPCR 预混液，具有 Hot-Start（热启动）特性。混合液中包含 SYBR® Green、dNTPs、Taq DNA 聚合酶以及惰性金色上样指示剂。通过 ScienCell 独特的化学修饰 Taq DNA 聚合酶实现的 Hot-Start 特性，可最大程度抑制引物二聚体形成。优化的缓冲体系提供更高的特异性和扩增效率，同时具有宽广的线性动态范围。惰性金色上样指示剂可在 qPCR 板或反应管加样时，清晰观察并跟踪样品加载情况。