人端粒绝对长度定量 qPCR 检测试剂盒

Telomeres are repetitive nucleotide elements at the ends of chromosomes that protect chromosomes from degradation and genetic information loss. Normal diploid cells lose telomeres with each cell cycle. Telomere length, therefore, decreases over time and may predict lifespan. Telomere shortening has negative effects on health conditions and has been linked to many health issues including aging and cancer. Accurate and consistent quantification of telomere length is important in many aspects of cell biology such as chromosomal instability, DNA repair, senescence, apoptosis, cell dysfunctions, and oncogenesis.
ScienCell's Absolute Human Telomere Length Quantification qPCR Assay Kit (AHTLQ) is designed to directly measure the average telomere length of a human cell population. The telomere primer set recognizes and amplifies telomere sequences. The single copy reference (SCR) primer set recognizes and amplifies a 100 bp-long region on human chromosome 17, and serves as reference for data normalization. The reference genomic DNA sample with known telomere length serves as a reference for calculating the telomere length of target samples. The carefully designed primers ensure: (i) high efficiency for trustworthy quantification; and (ii) no non-specific amplification. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis for amplification specificity and by template serial dilution for amplification efficiency. The 2X GoldNStart TaqGreen qPCR Master Mix (Cat #MB6018a-1) is a SYBR®Green dye-based qPCR master mix with a “hot-start” property. It contains SYBR®Green, dNTPs, Taq DNA polymerase, and an inert gold-color loading indicator in a single tube. The “hot-start” property achieved through ScienCell’s unique chemically modified Taq DNA polymerase provides maximal inhibition of primer dimer formation. The advanced buffer formulation provides superior specificity and efficiency with a wide linear dynamic range. The inert gold-color loading indicator allows for better visualization and tracking of sample loading in qPCR plates or tubes.

端粒（Telomeres）是位于染色体末端的重复核苷酸序列，其主要功能是保护染色体免受降解并防止遗传信息丢失。正常的二倍体细胞在每一次细胞周期中都会发生端粒缩短，因此端粒长度会随着时间逐渐减少，并可能成为预测寿命的重要指标。端粒缩短会对健康产生不利影响，并与衰老、癌症等多种疾病密切相关。对端粒长度进行准确且稳定的定量测定对于研究多种细胞生物学过程至关重要，例如染色体不稳定性、DNA 修复、细胞衰老、细胞凋亡、细胞功能异常以及肿瘤发生等。

ScienCell 的人端粒绝对长度定量 qPCR 检测试剂盒（Absolute Human Telomere Length Quantification qPCR Assay Kit，AHTLQ）可用于直接测定人类细胞群体的平均端粒长度。该试剂盒中的端粒引物组可特异性识别并扩增端粒序列；单拷贝参考基因（SCR）引物组可识别并扩增人类 17 号染色体上约 100 bp 的区域，作为数据归一化的参考。具有已知端粒长度的参考基因组 DNA 样本用于计算目标样本的端粒长度。

经过精心设计的引物可确保：高扩增效率，实现可靠的定量分析; 无非特异性扩增
每一组引物均通过 qPCR 熔解曲线分析（melt curve analysis）和凝胶电泳验证扩增特异性，并通过模板梯度稀释实验验证扩增效率。

试剂盒中包含 2X GoldNStart TaqGreen qPCR Master Mix（Cat# MB6018a-1），这是一种基于 SYBR® Green 染料的 qPCR 预混液，具有 Hot-Start（热启动）特性。该体系在单一反应管中包含 SYBR® Green、dNTPs、Taq DNA 聚合酶以及惰性金色上样指示剂。

通过 ScienCell 化学修饰的 Taq DNA 聚合酶实现的 Hot-Start 技术可最大程度抑制引物二聚体的形成。优化的缓冲体系提供更高的特异性和扩增效率，并具有宽广的线性动态范围。此外，惰性金色上样指示剂可在 qPCR 板或反应管加样时更清晰地观察和追踪样品加载情况。