Single Cell Whole Genome Amplification Kit

ScienCell’s Single Cell Whole Genome Amplification kit is designed for whole genome amplification from single cells, limited samples, or purified genomic DNA. The 5x RWGA master mix contains ScienCell’s unique thermal-stabled Phi 29 polymerase and optimized buffers and reagents. It allows for DNA amplification to be performed at a higher temperature, which provides maximal inhibition of GC content bias and nonspecific amplification. This product also allows the use of a flexible volume of starting sample and to scale-up depending on final yield requirement. The average product length of SCWGAK is in a range between 2 kb and 100 kb, enabling all downstream applications, such as long-range copy number variations, next-generation sequencing, array CGH genotyping or qPCR analysis. The genomic DNA yields is up to 20 µg per 30 µL reaction, depending on the quality of input sample (intact cells or DNA).