EmeraldNStart HiFi Marathon PCR Master Mix

ScienCell’s EmeraldNStart HiFi Marathon PCR Master Mix (ESPCRMM) is a 2X PCR master mix with a “hot-start” property. ESPCRMM is ideal for use in routine lab PCR reaction, and also for complex template or long-range PCR. The 2X master mix contains, dNTPs, ScienCell’s engineered DNA polymerase, inert green-color loading indicator and optimized buffer system. The error rate of ScienCell’s engineered DNA polymerase is over 25-fold lower than that of Taq polymerase. The “hot-start” property achieved through ScienCell’s unique chemically modified DNA polymerase provides maximal inhibition of primer dimer formation. The advanced buffer formulation provides superior specificity and efficiency to various templates (up to 20 kb for simple templates, and 10 kb for complex templates). The green inert loading indicator, a mixture of Orange G and Xylene Cyanol FF, allows for better visualization and tracking of sample loading in PCR tubes and directly to agarose gel. On a 1% agarose gel in 1x TAE, the loading indicator Xylene Cyanol (blue color) migrates at approximately 4 kb and Orange G (yellow color) migrates at approximately 50 bp.