Recombinant yeast SUMO Protease
SUMO (Small Ubiquitin-like MOdifiers) Protease 1 (Ulp1, UBI-specific protease 1 from Saccharomyces cerevisiae) is a highly active cysteine protease.
Unit Definition:
One unit of SUMO protease is defined as the amount of enzyme needed to cleave 90% of 100 µg of control SUMO tagged protein at 30°C for 1 hour.
SUMO (Small Ubiquitin-like MOdifiers) Protease 1 (Ulp1, UBI-specific protease 1 from Saccharomyces cerevisiae) is a highly active cysteine protease. Rather than recognizing a short sequence such as TEV, Thrombin or Enterokinease, SUMO protease specifically recognizes the tertiary structure of the hSUMO1-3 and yeast Smt3. SUMO fusion tag, as an N-terminal fusion partner, has been shown to enhance solubilized expression in the bacterial, yeast, insect cells or mammalian cells recombination protein expression systems. ScienCell's SUMO protease is fused with a N-terminal His-tag to facilitate its removal from the digested protein sample.
| 目录编号 | MB9018 |
|---|---|
| 制造国家 | 美国 |
| 产品编码 | rScUlp1 |
| 规格/数量 | 500 units, 2000 units |
| 产品用途 | 本产品仅供研究使用。未经批准,不得用于人体、动物或体外诊断程序。 |
| 储存 | 收到后,将 SUMO 蛋白酶(MB9018a)存放于 -80°C,将缓冲液(MB9018c 和 MB9018d)存放于 -20°C。首次解冻后分装。请避免反复冻融。妥善保存时,可稳定保存 24 个月。 |
| 运输 | 干冰。 |
| Source | Escherichia coli |
| Molecular Weight | 27 kDa |
| Biological Activity | One unit of SUMO protease is defined as the amount of enzyme needed to cleave 90% of 100 μg of control SUMO tagged protein at 30 ℃ for 1 hour. |
