EGF was originally discovered in crude preparations of nerve growth factor prepared from mouse submaxillary glands as an activity that induced early eyelid opening, incisor eruption, hair growth inhibition, and stunting of growth when injected into newborn mice. EGF is prototypic of a family of growth factors that are derived from membraneanchored precursors. All members of this family are characterized by the presence of at least one EGF structural unit (defined by the presence of a conserved 6 cysteine motif that forms three disulfide bonds) in their extracellular domain. EGF is initially synthesized as a 130 kDa precursor transmembrane protein containing 9 EGF units. The mature soluble EGF sequence corresponds to the EGF unit located proximal to the transmembrane domain. The membrane EGF precursor is capable of binding to the EGF receptor and was reported to be biologically active.
EGF最初是在小鼠下颌腺提取的粗神经生长因子制备物中发现的，当注射到新生小鼠体内时，可诱导早期眼睑开启、门牙萌出、抑制毛发生长以及生长受阻。EGF是一个以膜结合前体为基础的生长因子家族的典型代表。该家族的所有成员在其胞外结构域中至少含有一个EGF结构单元（由保守的6个半胱氨酸形成三条二硫键定义）。EGF最初合成为一条130 kDa的前体跨膜蛋白，包含9个EGF单元。成熟的可溶性EGF序列对应于靠近跨膜结构域的EGF单元。膜结合EGF前体能够结合EGF受体，并被报道具有生物活性。
| 目录编号 | 105-04 |
|---|---|
| 制造国家 | 美国 |
| 产品编码 | rhEGF |
| 规格/数量 | 100ug, 500ug, 1mg |
| 产品用途 | 本产品仅供研究使用。未经批准，不得用于人体、动物或体外诊断程序。 |
| 储存 | 收到后请在 -20°C 储存。复溶后可在 28°C 下保存最多一周。为确保最大稳定性，请分装并储存在 -20°C。避免反复冻融。 |
| 运输 | 凝胶包。 |
| Synonyms | Urogastrone, URG |
| AA Sequence | SNSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELR |
| Source | Escherichia coli |
| Molecular Weight | 6.2 kDa, a single non- glycosylated polypeptide chain containing 54 amino acids. |
| Purity | >95% |
| Physical Appearance | Light yellow to white powder |
| Formulation | Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH 7.4 with Dextran as protectant. |
| Endotoxin | <0.1 ng/μg of protein (<1 EU/μg) |
| Reconstitution | Reconstitute in sterile distilled water containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. |
