Ready-to-use 3D Human Preadipocyte Spheroids

Adipocytes are specialized cells that play a critical role in various metabolic events. Under physiological conditions, adipocytes serve as an energy storage reservoir, as well as exert endocrine effects through the secretion of cytokines and adipokines. Dysregulation in the homeostatic function of adipose tissue results in a plethora of metabolic diseases such as type II diabetes and cardiovascular diseases. Obesity is a risk factor for type II diabetes as hypertrophy of adipose tissue may contribute to insulin resistance. To better understand the physiology of adipocytes in the diseased state and to evaluate potential therapeutic targets for these events, models that appropriately resemble fat tissue is vital. 2D monolayer culture of adipocytes in vitro is useful but is limited in its inability to reflect the complexity of human fat tissue. To provide a more native in vitro adipose model, ScienCell offers a ready-to-use human preadipocyte spheroid (SP3D-HPA) that can be easily differentiated into mature adipocytes in culture. Upon differentiation, these adipocytes accumulate large lipid droplets confirmed by Oil Red O staining. This spheroid model serves as an excellent tool for drug discovery and the study of adipocyte biology.