Ready-to-use 3D Human Chondrocyte-articular Spheroid Kit

The composition and structural integrity of extracellular matrix (ECM) within the cartilage is responsible for enduring the tensile strength of joint biomechanics. Chondrocytes, the main constituent of cartilage, mediate the synthesis and degradation of ECM macromolecules (such as Type II collagen and aggrecan) within the matrix. Type II collagen and aggrecan provide the tensile strength and the osmotic resistance for cartilage, respectively. Chondrocytes in monolayer culture, however, are susceptible to dedifferentiation. Thus, an improved cell culture model that closely mimics the in vivo environment is necessary to maintain the features of differentiated chondrocytes. ScienCell has developed a highly innovative ready-to-use 3D chondrocyte spheroid kit (SP3D-HCaS) that better approximates the in vivo environment. Using ScienCell’s Ready-to-use 3D spheroid kit, researchers can obtain highly homogenous and functional 3D spheroids in 24 hours after thawing, without encountering the long and complex workflow of 3D culture.

In 3D spheroid culture, we confirmed that the chondrocytes maintain functional markers such as type II collagen, aggrecan, and Sox9 as determined by qPCR analysis. ScienCell’s SP3D-HCaS provides an excellent in vitro model for studying normal chondrocyte physiology, mechanism of degenerative joint diseases, and cartilage tissue repair and engineering.