All-inclusive 3D Human Chondrocyte-articular Spheroid Formation Kit

The composition and structural integrity of extracellular matrix (ECM) within the cartilage is responsible for enduring the tensile strength of joint biomechanics. Chondrocytes, the main constituent of cartilage, mediate the synthesis and degradation of ECM macromolecules (such as Type II collagen and aggrecan) within the matrix. Type II collagen and aggrecan provide the tensile strength and the osmotic resistance for cartilage, respectively. Chondrocytes in monolayer culture, however, are susceptible to dedifferentiation. Thus, an improved cell culture model that closely mimics the in vivo environment is necessary to maintain the features of differentiated chondrocytes. ScienCell has developed an all-inclusive 3D chondrocyte spheroid formation kit (3D-HCaSF) enabling the growth of human primary chondrocytes in a more biologically relevant 3D environment. By growing in a proper three-dimensional environment in vitro, chondrocytes maintain functional markers such as type II collagen, aggrecan, and Sox9 as validated by the qPCR analysis. ScienCell’s 3D-HCaSF provides an excellent in vitro model for studying normal chondrocyte physiology, mechanism of degenerative joint diseases, and cartilage tissue repair and engineering.