大鼠皮层神经元

The tissue of the central nervous system is made up of two classes of cells that may be broadly categorized as neurons and glia. Neurons are anatomic, functional, and trophic units of the brain. Neurons are dynamically polarized cells responsible for electrochemically transmitting information throughout the nervous system. Despite great variability in size and shape, all neurons share common morphological features, the key elements of a highly complex communication network. In rat, the adult brain has been shown to contain 200 million neurons. The outermost layers of the brain are the cerebral cortex, home to numerous critical functions including perception, language, decision making, and memory. Primary rat neurons-cortical (RNc) are a useful in vitro model for studying conditions such as Alzheimer’s disease, dementia and traumatic brain injury. RN-c from ScienCell Research Laboratories are isolated from embryonic day 18 CD® IGS rat cerebrum. RN-c are cryopreserved at P0 and delivered frozen. Each vial contains >1 x 10⁶ cells in 1 ml volume.

RN-c are characterized by immunofluorescence with antibodies specific to β-tubulin III. RN-c are negative for mycoplasma, bacteria, yeast, and fungi. RN-c are guaranteed to further culture under the conditions provided by ScienCell Research Laboratories; however, RN-c are not recommended for expanding or long-term cultures since the cells do not proliferate in culture.

Recommended Medium
It is recommended to use neuronal medium (NM, Cat. #1521) for culturing RN-c in vitro.

中枢神经系统组织由两类细胞组成，通常可大致分为神经元和神经胶质细胞。神经元是大脑的解剖学、功能学及营养学基本单位，是动态极化细胞，负责在整个神经系统中进行电化学信息传递。尽管神经元在大小和形态上具有高度多样性，但它们共享基本的形态学特征，构成高度复杂的通信网络的关键组成部分。在大鼠中，成年大脑约含2亿个神经元。

大脑最外层为大脑皮层（cerebral cortex），参与多种关键功能，包括感知、语言、决策和记忆等。原代大鼠皮层神经元（RN-c）是研究阿尔茨海默病、痴呆及创伤性脑损伤等疾病的重要体外模型。

ScienCell研究实验室提供的大鼠皮层神经元（RN-c）来源于胚胎第18天（E18）CD® IGS大鼠大脑组织（cerebrum）分离获得。RN-c细胞于P0代冻存，并以冷冻状态提供。每支冻存管含>1 × 10⁶个细胞，冻存体积为1 mL。

RN-c通过针对β-微管蛋白III（β-tubulin III）的特异性抗体进行免疫荧光鉴定。经检测，RN-c不含支原体、细菌、酵母及真菌污染。在ScienCell提供的培养条件下可维持存活，但不建议进行扩增或长期培养，因为该细胞在体外不具备增殖能力。

推荐培养基

建议使用神经元培养基（Neuronal Medium，NM，产品编号：1521）进行RN-c体外培养。