C57BL/6来源小鼠皮层神经元

The tissue of the central nervous system is made up of two classes of cells that may be broadly categorized as neurons and glia. Neurons are anatomic, functional, and trophic units of the brain. Neurons are dynamically polarized cells responsible for electrochemically transmitting information throughout the nervous system. Despite great variability in size and shape, all neurons share common morphological features, the key elements of a highly complex communication network. The outermost layers of the brain are the cerebral cortex, home to numerous critical functions including perception, language, decision making, and memory. Primary mouse neurons-cortical (MN-c) are a useful in vitro model for studying conditions such as Alzheimer’s disease, dementia and traumatic brain injury.

MN-c from ScienCell Research Laboratories are isolated from embryonic day 18 C57BL/6 mouse cerebrum. MN-c are cryopreserved at P0 and delivered frozen. Each vial contains >1 x 10⁶ cells in 1 ml volume. MN-c are characterized by immunofluorescence with antibodies specific to β-tubulin III. MN-c are negative for mycoplasma, bacteria, yeast, and fungi. MN-c are guaranteed to further culture under the conditions provided by ScienCell Research Laboratories; however, MN-c are not recommended for expanding or long-term cultures since the cells do not proliferate in culture.

Recommended Medium
It is recommended to use neuronal medium (NM, Cat. #1521) for culturing MN-c in vitro.

中枢神经系统组织主要由两大类细胞组成，即神经元和胶质细胞。神经元是大脑的解剖、功能和营养支持基本单位。神经元具有动态极性，能够通过电化学信号在整个神经系统中传递信息。尽管神经元在大小和形态上存在显著差异，但它们均具有共同的形态学特征，是构成高度复杂信息交流网络的基本要素。大脑最外层为大脑皮层，参与感觉、语言、决策和记忆等多项重要功能。原代小鼠皮层神经元（MN-c）是一种有用的体外研究模型，可用于研究阿尔茨海默病、痴呆和创伤性脑损伤等疾病及相关病理状态。

ScienCell Research Laboratories提供的小鼠皮层神经元（MN-c）分离自胚胎第18天（E18）C57BL/6小鼠的大脑。MN-c在第0代（P0）时进行冻存，并以冷冻状态运输。每瓶含有超过1 × 10⁶个细胞，体积为1 ml。MN-c采用β-III型微管蛋白（β-tubulin III）特异性抗体进行免疫荧光鉴定。经检测，MN-c不含支原体、细菌、酵母和真菌。按照ScienCell Research Laboratories提供的培养条件，MN-c可继续进行体外培养；但是，由于MN-c在培养过程中不会增殖，因此不建议用于细胞扩增或长期培养。

推荐培养基
推荐使用神经元培养基（NM，产品编号1521）进行MN-c的体外培养。

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