大鼠心脏成纤维细胞

Cardiac fibroblasts (CF) are the principal cell type of the heart. They provide structural support for cardiac myocytes, are a source of paracrine growth factors, and are an important cellular component of the myocardial response to injury. CF are responsible for extracellular matrix synthesis in the heart during development and pathological conditions. Their proliferation and matrix synthesis is essential for scar formation at sites of myocardial infarction, cardiac fibrosis, and is often complicated by cardiac hypertrophy. CF also respond to changes in microenvironments in pathological conditions by modulating integrin expression. CF culture has been widely used as a model to study the cardiac matrix remodeling by physiological (exercise) and pathological (hypertension) stressors.

RCF from ScienCell Research Laboratories are isolated from CD® IGS rat heart. RCF are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. RCF are characterized by fibroblast morphology and immunofluorescence with antibody specific to fibronectin. RCF are negative for mycoplasma, bacteria, yeast, and fungi. RCF are guaranteed to further expand for 5 population doublings under the conditions provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Fibroblast Medium-2 (FM-2, Cat. #2331) for culturing RCF in vitro.

心脏成纤维细胞（Cardiac Fibroblasts，CF）是心脏中的主要细胞类型之一。它们为心肌细胞提供结构支持，是旁分泌生长因子的来源，并在心肌损伤应答过程中发挥重要作用。CF 负责心脏发育及病理状态下细胞外基质（ECM）的合成，其增殖和基质合成对于心肌梗死区域瘢痕形成、心脏纤维化以及伴随发生的心肌肥厚至关重要。在病理条件下，CF 还可通过调节整合素（Integrin）的表达响应微环境变化。CF 培养已被广泛用于研究生理性应激（如运动）和病理性应激（如高血压）诱导的心脏基质重塑机制。

ScienCell Research Laboratories 提供的大鼠心脏成纤维细胞（RCF）分离自 CD^® IGS 大鼠心脏组织。RCF 于第 1 代（P1）进行冻存，并以冻存形式提供。每瓶含有 >5 × 10⁵ 个细胞，悬浮于 1 ml 冻存液中。RCF 通过成纤维细胞形态学特征及纤维连接蛋白（Fibronectin）特异性抗体免疫荧光进行鉴定。RCF 经检测不含支原体、细菌、酵母和真菌污染。ScienCell 保证 RCF 在推荐培养条件下可继续扩增至少 5 个群体倍增。

推荐培养基

建议使用成纤维细胞培养基-2（Fibroblast Medium-2，FM-2，产品编号：2331）进行 in vitro RCF 培养。