大鼠脑血管周细胞

Brain Vascular Pericytes (BVP) are perivascular cells that are closely associated with the endothelium of capillaries and other small vessels. BVP, located between endothelial cells and astrocytes in the brain, communicate with other cells by extending long cytoplasmic processes which wrap around the capillaries. BVP participate in a variety of processes including angiogenesis, endothelial cell survival, regulation of capillary blood flow, and establishment and maintenance of the blood-brain barrier. Pericyte dysregulation has been linked to several pathological conditions such as hypertension, diabetic retinopathy, atherosclerosis, multiple sclerosis, Alzheimer's disease, and tumor angiogenesis. The unique and diverse functions of BVP make them novel candidates for cell therapy in regenerative medicine. Cultured primary rat BVP (RBVP) are a useful in vitro model for understanding the molecular mechanisms of blood-brain barrier regulation and for studying a wide variety of central nervous system diseases.

RBVP from ScienCell Research Laboratories are isolated from adult CD® IGS rat brain. RBVP are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. RBVP are characterized by immunofluorescence with antibody specific to platelet-derived growth factor-β (PDGF receptor-β) and α-smooth muscle actin. RBVP are negative for mycoplasma, bacteria, yeast, and fungi. RBVP are guaranteed to further expand for 5 population doublings under the conditions provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Pericyte Medium (PM, Cat. #1201) for culturing RBVP in vitro.

脑血管周细胞（Brain Vascular Pericytes，BVP）是一类位于毛细血管及其他微小血管周围、与内皮细胞紧密相关的周血管细胞。

在脑组织中，BVP 位于内皮细胞与星形胶质细胞之间，并通过伸展包绕毛细血管的长细胞质突起与其他细胞进行相互通讯。

BVP 参与多种重要生理过程，包括血管生成、内皮细胞存活调控、毛细血管血流调节以及血脑屏障的建立与维持。

血管周细胞功能失调与多种疾病密切相关，包括高血压、糖尿病性视网膜病变、动脉粥样硬化、多发性硬化、阿尔茨海默病以及肿瘤血管生成等。

BVP 独特且多样化的生物学功能，使其成为再生医学领域具有潜力的细胞治疗候选细胞。培养的大鼠原代脑血管周细胞（RBVP）是研究血脑屏障调控分子机制以及多种中枢神经系统疾病的重要 in vitro 模型。

ScienCell Research Laboratories 提供的大鼠脑血管周细胞（RBVP）分离自成年 CD® IGS 大鼠脑组织。细胞于第一代（Passage 1）完成冻存，并以冷冻状态运输。每瓶产品含有超过 5 × 10⁵ 个细胞，包装体积为 1 ml。

RBVP 通过针对血小板源性生长因子受体-β（PDGF receptor-β）及 α-平滑肌肌动蛋白（α-smooth muscle actin）的特异性抗体进行免疫荧光鉴定。经检测，产品不含支原体、细菌、酵母及真菌污染。在按照 ScienCell Research Laboratories 提供的培养条件操作时，可保证细胞至少完成 5 次群体倍增。

推荐培养基

建议使用血管周细胞培养基（Pericyte Medium，PM，产品编号 #1201）进行 RBVP 的 in vitro 培养。