兔肺成纤维细胞

The most abundant cell type in the lung interstitium is the fibroblast. These fibroblasts resemble ordinary fibroblasts, but also possess some distinguishing features such as long, branching processes and gap junctions. Pulmonary fibroblasts function to produce type III collagen, elastin, and proteoglycans of the extracellular matrix of the alveolar septa. They play an important role in the repair and remodeling processes following tissue damage. The controlled accumulation of fibroblasts to sites of inflammation is crucial to effective tissue repair after injury. An inadequate or an excessive accumulation of fibroblasts can result in abnormal tissue function. For example, excess proliferation of fibroblasts contributes to adventitial thickening observed during the development of hypoxia-induced pulmonary hypertension. Rabbit pulmonary fibroblasts (RabPF) are a useful model for elucidating the mechanisms of pulmonary disease.

RabPF from ScienCell Research Laboratories are isolated from rabbit lung. RabPF are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. RabPF are characterized by immunofluorescence with antibody specific to fibronectin. RabPF are negative for mycoplasma, bacteria, yeast, and fungi. RabPF are guaranteed to further expand for 10 population doublings under the conditions provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Fibroblast Medium (FM, Cat. #2301) for culturing RabPF in vitro.

肺间质中最丰富的细胞类型为成纤维细胞。这类细胞在形态上类似普通成纤维细胞，但也具有一些独特特征，例如较长的分支状突起以及缝隙连接。肺成纤维细胞主要功能是合成肺泡隔细胞外基质中的Ⅲ型胶原、弹性蛋白以及蛋白聚糖。它们在组织损伤后的修复与重塑过程中发挥重要作用。在炎症部位对成纤维细胞的受控募集是实现有效组织修复的关键。然而，成纤维细胞的不足或过度积累均可能导致异常组织功能。例如，成纤维细胞的过度增殖参与了缺氧性肺动脉高压发生过程中所观察到的外膜增厚。兔肺成纤维细胞（RabPF）是用于阐明肺部疾病机制的有价值模型。

RabPF 由 ScienCell Research Laboratories 提供，分离自兔肺组织。RabPF 在第一代（P1）时进行冷冻保存，并以冻存状态交付。每瓶含有 >5 × 10⁵ 个细胞，体积为 1 ml。RabPF 通过针对纤维连接蛋白（fibronectin）的特异性抗体进行免疫荧光鉴定。经检测，RabPF 不含支原体、细菌、酵母及真菌污染。在 ScienCell Research Laboratories 提供的培养条件下，RabPF 保证可进一步扩增至 10 代倍增。

推荐培养基

建议使用 Fibroblast Medium（FM，产品编号 #2301）进行 RabPF 的 体外（in vitro） 培养。