兔心脏成纤维细胞

Cardiac fibroblasts (CF) provide structural support for cardiac myocytes and are responsible for extracellular matrix synthesis in the heart during growth and pathophysiological conditions. CF are an important cellular component of myocardial responses to injury and the source of paracrine growth factors. CF proliferation and synthesis of matrix is essential for scar formation at sites of myocardial infarction and cardiac fibrosis. CF cultures have been widely used as a model to study the cardiac matrix remodeling by physiological (exercise) and pathological (hypertension) stressors.

RabCF from ScienCell Research Laboratories are isolated from rabbit heart. RabCF are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. RabCF are characterized by immunofluorescence with antibody specific to fibronectin. RabCF are negative for mycoplasma, bacteria, yeast, and fungi. RabCF are guaranteed to further expand for 10 population doublings under the conditions provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Fibroblast Medium-2 (FM-2, Cat. #2331) for culturing RabCF in vitro.

心脏成纤维细胞（Cardiac Fibroblasts，CF）为心肌细胞提供结构支持，并负责心脏在生长发育及病理生理状态下细胞外基质（ECM）的合成。CF 是心肌损伤应答过程中的重要细胞组成，也是旁分泌生长因子的来源。CF 的增殖及基质合成对于心肌梗死区域的瘢痕形成和心脏纤维化过程至关重要。CF 培养已被广泛用于研究生理性应激（如运动）和病理性应激（如高血压）诱导的心脏基质重塑机制。

ScienCell Research Laboratories 提供的兔心脏成纤维细胞（RabCF）分离自兔心脏组织。RabCF 于第 1 代（P1）进行冻存，并以冻存形式提供。每瓶含有 >5 × 10⁵ 个细胞，悬浮于 1 ml 冻存液中。RabCF 经纤维连接蛋白（Fibronectin）特异性抗体免疫荧光鉴定，且经检测不含支原体、细菌、酵母和真菌污染。ScienCell 保证 RabCF 在推荐培养条件下可继续扩增至少 10 个群体倍增。

推荐培养基

建议使用成纤维细胞培养基-2（Fibroblast Medium-2，FM-2，产品编号：2331）进行 in vitro RabCF 培养。