猪视网膜星形胶质细胞

Astrocytes are the major cell type in the mammalian brain. They provide a variety of supportive functions to their partner neurons in the central nervous system (CNS), such as neuronal guidance during development, nutritional and metabolic support throughout life. Numerous studies have demonstrated that astrocytes are among the most functionally diverse group of cells in the CNS. Impairment of normal astrocyte functions during stroke and other insults can critically influence neuron survival. Long-term recovery after brain injury, through neurite outgrowth, synaptic plasticity, or neuron regeneration, is also influenced by astrocyte surface molecule expression and trophic factor release. Apart from various neuropathological conditions, astrocytes play a particularly important role in retinal vascular disease and glaucoma. Much of what we have learned about astrocytes is from in vitro studies and astrocyte culture is a useful tool for exploring the diverse properties of this cell type.

PA-r from ScienCell Research Laboratories are isolated from porcine retina. PA-r are cryopreserved at passage one and delivered frozen. Each vial contains >1 x 10⁶ cells in 1 ml volume. PA-r are characterized by immunofluorescence with antibody specific to GFAP. PA-r are negative for mycoplasma, bacteria, yeast, and fungi. PA-r are guaranteed to further expand for 5 population doublings under the conditions provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Astrocyte Medium-animal (AM-a, Cat. #1831) for culturing PA-r in vitro.

星形胶质细胞是哺乳动物脑内的主要胶质细胞类型之一，在中枢神经系统（CNS）中为神经元提供多种支持功能，包括在发育过程中引导神经元迁移与轴突生长，以及在整个生命过程中为神经元提供营养和代谢支持。大量研究表明，星形胶质细胞是中枢神经系统中功能多样性最丰富的细胞群之一。在卒中及其他脑损伤情况下，星形胶质细胞正常功能受损可能显著影响神经元的存活。脑损伤后的长期恢复也受到星形胶质细胞的影响，其作用机制包括调节细胞表面分子的表达及营养因子的释放，从而影响神经突生长、突触可塑性和神经元再生。除参与多种神经病理过程外，星形胶质细胞在视网膜血管疾病和青光眼的发生发展中也发挥着重要作用。我们对星形胶质细胞的许多认识来自体外研究，因此，星形胶质细胞培养是探索此类细胞多样化生物学特性与功能的重要实验工具。

ScienCell Research Laboratories 提供的猪视网膜星形胶质细胞（PA-r）分离自猪视网膜。细胞以第1代（P1）冻存，并以冷冻状态运输。每瓶含有超过 1 x 10⁶ 个细胞，悬液体积为 1 ml。PA-r 采用针对胶质纤维酸性蛋白（GFAP）的特异性抗体进行免疫荧光鉴定。经检测，PA-r 不含支原体、细菌、酵母菌及真菌。在遵循 ScienCell Research Laboratories 提供的培养条件进行培养的情况下，PA-r 保证可继续扩增至少 5 个群体倍增（population doublings）。

推荐培养基

建议使用动物源星形胶质细胞培养基（Astrocyte Medium-animal，AM-a，产品编号：1831）进行 PA-r 的体外培养。

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