pNPP Phosphatase Assay
PNPP is suitable to detect acid and neutral phosphatase activity. The Assay Buffer- neutral (Cat #8108a), with a pH of 7.2, is compatible with most neutral phosphatases, such as protein phosphatases. The Assay Buffer-acidic (Cat #8108b), with a pH of 5.5, is optimized for acid phosphatase activity. PNPP is used to evaluate phosphatase activity in vitro and is for research use only. It is not approved for human or animal use or for applications in clinical or in vitro diagnostic procedures.
Protein phosphatase, an enzyme that catalyzes the removal of phosphate (PO43- ) groups from protein molecules, plays a crucial role in regulating essential cellular processes such as cell attachment, proliferation, differentiation, and apoptosis. The ScienCell™ pNPP Phosphatase Assay (PNPP) is designed to detect phosphatase activity in biological samples, utilizing para-nitrophenyl phosphate (pNPP) as a chromogenic substrate. Upon hydrolysis, pNPP yields para-nitrophenol, which forms a vibrant yellow solution under alkaline conditions. This colorimetric change can be accurately measured using a spectrophotometer at a wavelength of 405 nm. The enzyme provided in the kit is an acid phosphatase derived from potato.
| 目录编号 | 8108 |
|---|---|
| 制造国家 | 美国 |
| 产品编码 | PNPP |
| 规格/数量 | 500 Tests |
| 产品用途 | 本产品仅供研究使用。未经批准，不得用于人体、动物或体外诊断程序。 |
| 储存 | 收到后，请将 #8108a、#8108b、#8108d、#8108e 和 #8108g 存放于 4°C，#8108c 和 #8108f 请避光存放于 -20°C。 |
| 运输 | 干冰。 |