来源于 C57BL/6 小鼠的脑膜细胞

Meningeal cells surround the brain and actively participate in the development of the central nervous system. They play an important role in stabilizing the extracellular matrix of the pial surface, organizing the radial glial scaffold, and laminating the cerebellar cortex. Selective pharmacological destruction of the meningeal cells during a critical ontogenetic period leads to specific malformation of both the cerebellar cortex and dentate gyrus. Grafts of meningeal cells, which are derived from meninges overlying the cerebral cortex, in adult rat spinal cord lesions promotes axonal regrowth. Additionally, in vitro studies showed that meningeal cells chemotactically orient the migration of immature neurons but not glial cells.

MMC from ScienCell Research Laboratories are isolated from C57BL/6 mouse leptomeningi. MMC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. MMC are characterized by immunofluorescence; they are positive for fibronectin and negative for GFAP. MMC are negative for mycoplasma, bacteria, yeast, and fungi. MMC are guaranteed to further expand for 5 population doublings under the conditions specified by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Meningeal Cell Medium (MenCM, Cat. #1401) for culturing MMC in vitro.

脑膜细胞包绕脑组织，并积极参与中枢神经系统的发育。脑膜细胞在稳定软脑膜表面的细胞外基质、组织放射状胶质细胞支架以及小脑皮质分层过程中发挥重要作用。在关键的个体发育时期选择性地通过药理学方法破坏脑膜细胞，会导致小脑皮质和齿状回发生特定的畸形。在成年大鼠脊髓损伤模型中，将来源于覆盖大脑皮质的脑膜组织的脑膜细胞进行移植，可促进轴突再生。此外，体外研究表明，脑膜细胞可通过趋化作用引导未成熟神经元的迁移，但不会引导胶质细胞的迁移。

ScienCell Research Laboratories 的小鼠脑膜细胞（MMC）分离自 C57BL/6 小鼠软脑膜。MMC 在第 1 代时进行冻存，并以冷冻状态运输。每瓶含有超过 5 × 10⁵ 个细胞，体积为 1 ml。MMC 通过免疫荧光进行鉴定；纤连蛋白（fibronectin）呈阳性，GFAP 呈阴性。MMC 经检测不含支原体、细菌、酵母和真菌。在 ScienCell Research Laboratories 规定的培养条件下，MMC 保证可进一步扩增 5 个群体倍增。

推荐培养基

建议使用 Meningeal Cell Medium（MenCM，产品编号 #1401）在体外培养 MMC。

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