CD1 小鼠真皮成纤维细胞

The skin is composed of two layers: the epidermis and the dermis. Dermal fibroblasts are located in the dermis and play an active role in wound healing and production of extracellular matrix. Fibroblasts secrete a non-rigid extracellular matrix which is rich in type I and/or type III collagen. Dermal fibroblasts have the ability to switch from a proliferative, migratory phase to a contractile, matrix-remodeling phase during wound healing. The plasticity of dermal fibroblasts is vital for wound healing, however, it can also lead to the development of skin disease. Dermal fibroblasts also provide a critical function in skin homeostasis and aging. Mouse dermal fibroblasts (MDF) can be used to further study skin disease, skin aging, and the wound healing process.

MDF from ScienCell Research Laboratories are isolated from postnatal day 2 CD-1 mouse skin. MDF are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. MDF are characterized by their spindle morphology and immunofluorescence with antibody specific to fibronectin. MDF are negative for mycoplasma, bacteria, yeast, and fungi. MDF are guaranteed to further expand for 5 population doublings under the conditions provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Fibroblast Medium-2 (FM-2, Cat. #2331) for culturing MDF in vitro.

皮肤由两层组成：表皮和真皮。真皮成纤维细胞位于真皮层，在伤口愈合以及细胞外基质的生成中发挥重要作用。成纤维细胞会分泌富含I型和/或III型胶原蛋白的非刚性细胞外基质。在伤口愈合过程中，真皮成纤维细胞能够从增殖和迁移阶段转变为收缩及基质重塑阶段。真皮成纤维细胞的这种可塑性对于伤口愈合至关重要，但同时也可能导致皮肤疾病的发生。真皮成纤维细胞在皮肤稳态维持及衰老过程中同样具有关键作用。小鼠真皮成纤维细胞（MDF）可用于进一步研究皮肤疾病、皮肤衰老以及伤口愈合过程。

ScienCell Research Laboratories 提供的MDF分离自出生后第2天（postnatal day 2）的CD-1小鼠皮肤。MDF在第一代（passage one）时进行冷冻保存，并以冻存形式交付。每瓶含有超过5 × 10⁵个细胞，总体积为1 ml。MDF通过梭形成纤维样形态以及针对纤维连接蛋白（fibronectin）的特异性抗体免疫荧光进行鉴定。MDF经检测不含支原体、细菌、酵母及真菌。在ScienCell Research Laboratories提供的培养条件下，保证MDF可进一步扩增5次群体倍增（population doublings）。

推荐培养基

建议使用 Fibroblast Medium-2（FM-2，货号 #2331）用于MDF的 in vitro 培养。