C57BL/6 小鼠心脏成纤维细胞
C57BL/6 小鼠心脏成纤维细胞（MCF）分离自出生后第 2 天或第 8 天的 C57BL/6 小鼠心脏组织。MCF 于 P0 代冻存，并以冻存形式提供。每瓶含有 >5 × 105 个细胞，悬浮于 1 ml 冻存液中。
The cardiac fibroblasts (CF) are the principal cell type of the heart. They provide structural support for cardiac myocytes, are a source of paracrine growth factors, and are an important cellular component of myocardial responses to injury. CF are responsible for extracellular matrix synthesis in the heart during development and pathological conditions. Their proliferation and matrix synthesis is essential for scar formation at sites of myocardial infarction, cardiac fibrosis, and is often complicated by cardiac hypertrophy. CF also respond to changes in microenvironments in pathological conditions by modulating integrin expression. CF culture has been widely used as a model to study the cardiac matrix remodeling by physiological (exercise) and pathological (hypertension) stressors.
MCF from ScienCell Research Laboratories are isolated from postnatal day 2 or postnatal day 8 C57BL/6 mouse heart. MCF are cryopreserved at P0 and delivered frozen. Each vial contains >5 x 105 cells in 1 ml volume. MCF are characterized by immunofluorescence with antibody specific to fibronectin. MCF are negative for mycoplasma, bacteria, yeast, and fungi. MCF are guaranteed to further culture under the conditions provided by ScienCell Research Laboratories; however, MCF are not recommended for long-term cultures due to limited expansion capacity and senescence after subculturing.
Recommended Medium
It is recommended to use Fibroblast Medium-2 (FM-2, Cat. #2331) for culturing MCF in vitro.
心脏成纤维细胞（Cardiac Fibroblasts，CF）是心脏中最主要的细胞类型之一。它们为心肌细胞提供结构支持，是旁分泌生长因子的来源，并在心肌损伤应答中发挥重要作用。CF 负责心脏发育及病理状态下细胞外基质（ECM）的合成，其增殖和基质合成对于心肌梗死后瘢痕形成、心脏纤维化及心肌肥厚的发展至关重要。在病理条件下，CF 还可通过调节整合素（Integrin）的表达来响应微环境变化。由于其在心脏基质重塑中的关键作用，CF 培养已广泛应用于研究生理性应激（如运动）和病理性应激（如高血压）诱导的心脏重塑机制。
ScienCell Research Laboratories 提供的 C57BL/6 小鼠心脏成纤维细胞（MCF）分离自出生后第 2 天或第 8 天的 C57BL/6 小鼠心脏组织。MCF 于 P0 代冻存，并以冻存形式提供。每瓶含有 >5 × 105 个细胞，悬浮于 1 ml 冻存液中。MCF 经纤维连接蛋白（Fibronectin）特异性抗体免疫荧光鉴定，且经检测不含支原体、细菌、酵母和真菌污染。ScienCell 保证 MCF 在推荐培养条件下能够继续培养；但由于传代后的增殖能力有限且易发生细胞衰老，不建议用于长期培养。
推荐培养基
建议使用成纤维细胞培养基-2（Fibroblast Medium-2，FM-2，产品编号：2331）进行 in vitro MCF 培养。
| 目录编号 | M6300-57 |
|---|---|
| 制造国家 | 美国 |
| 产品编码 | MCF |
| 规格/数量 | 5 x 10^5 cells/vial |
| 产品用途 | 本产品仅供研究使用。未经批准，不得用于人体、动物或体外诊断程序。 |
| 储存 | 收到后，立即将细胞从干冰转移到液氮中，并在液氮中保存，直到需要进行实验。 |
| 运输 | 干冰。 |
