C57BL/6小鼠脊髓星形胶质细胞

Astrocytes are the major cell type in the mammalian brain. They have been implicated in a variety of supportive functions for their partner neurons in the central nervous system, such as neuronal guidance during development, ion and water homeostasis, blood flow regulation, neurotransmission, energy metabolism, and immune defense. Recent studies have shown that spinal cord astrocytes contribute to neuroinflammation by chemokine expression, which leads to the recruitment of “inflammatory” monocytes and neutrophils to the lesion site. Experimentally, spinal cord astrocytes have also been used to study the wobbler mutation and astrogliosis. As the importance of astrocyte function in the nervous system becomes more recognized, particularly in regards to the modulation of neural activity, astrocyte cultures will provide a useful tool for exploring the diverse properties and functions of these cells. 

MA-sc from ScienCell Research Laboratories are isolated from embryonic day 14 C57BL/6 mouse spinal cord. MA-sc are cryopreserved at P0 and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. MA-sc are characterized by immunofluorescence with antibody specific to GFAP. MA-sc are negative for mycoplasma, bacteria, yeast, and fungi. MA-sc are guaranteed to further expand for 5 population doublings under the condition provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Astrocyte Medium-animal (AM-a, Cat. #1831) for culturing MA-sc in vitro.

星形胶质细胞是哺乳动物脑内的主要胶质细胞类型之一，在中枢神经系统中发挥多种重要的支持和调节作用，包括在神经系统发育过程中引导神经元迁移与轴突生长、维持离子和水稳态、调节脑血流、参与神经递质的代谢与循环、支持能量代谢以及参与免疫防御。近期研究表明，脊髓星形胶质细胞可通过表达趋化因子参与神经炎症反应，促进炎症性单核细胞和中性粒细胞向损伤部位募集。在实验研究中，脊髓星形胶质细胞也被用于研究 wobbler 突变相关的神经病理变化及星形胶质细胞增生（astrogliosis）。随着研究的深入，人们逐渐认识到星形胶质细胞在神经系统中的功能远不止于支持神经元，尤其是在调节神经活动方面发挥着重要作用。因此，星形胶质细胞培养物可作为研究其多样化生物学特性与功能的重要实验工具。

ScienCell Research Laboratories 提供的小鼠脊髓星形胶质细胞（MA-sc）分离自胚胎第14天的 C57BL/6 小鼠脊髓。细胞以 P0 代冻存，并以冷冻状态运输。每瓶含有超过 5 x 10⁵ 个细胞，悬液体积为 1 ml。MA-sc 经免疫荧光检测，并使用针对胶质纤维酸性蛋白（GFAP）的特异性抗体进行鉴定。经检测，MA-sc 不含支原体、细菌、酵母菌及真菌。在遵循 ScienCell Research Laboratories 提供的培养条件进行培养的情况下，MA-sc 保证可继续扩增至少 5 个群体倍增（population doublings）。

推荐培养基

建议使用动物源星形胶质细胞培养基（Astrocyte Medium-animal，AM-a，产品编号：1831）进行 MA-sc 的体外培养。

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