人扁桃体上皮细胞
ScienCell Research Laboratories 的人扁桃体上皮细胞（HTEpiC）分离自人扁桃体组织。HTEpiC 以第 1 代（P1）冻存，并以冷冻状态运输。每瓶含有 >5 × 105 个细胞，体积为 1 ml。
Tonsil epithelium is a heterogeneous lymphoid organ containing two different types of actively differentiating epithelia, the lining stratified squamous epithelium and the reticulated crypt, or lymphoepithelium. The tonsil crypts represent a specialized compartment, important in the immunological functions of the tonsil because of the immediate proximity of the epithelial and lymphoid tissues. In situ staining of tonsil epithelium suggests that expression of the simple epithelial keratins K8, K18 and K19 is located to the crypt epithelial cells. Cell culture of tonsil epithelium produces heterogeneous squamous epithelial cells in which differentiation appears to be ongoing and keratin expression patterns are consistent with those found in tonsil epithelial cells in situ.
HTEpiC from ScienCell Research Laboratories are isolated from human tonsil. HTEpiC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 105 cells in 1 ml volume. HTEpiC are characterized by immunofluorescence with antibodies specific to cytokeratin18 and/or cytokeratin-19. HTEpiC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HTEpiC are guaranteed to further expand for 15 population doublings under the conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Tonsil Epithelial Cell Medium (TEpiCM, Cat #2561) for culturing HTEpiC in vitro.
扁桃体上皮是一个具有高度异质性的淋巴组织器官，其中包含两种具有持续分化能力的上皮组织：覆盖于表面的复层鳞状上皮，以及形成网状结构的隐窝上皮（又称淋巴上皮）。扁桃体隐窝是一个特殊的功能区域，由于上皮组织与淋巴组织紧密相邻，在扁桃体的免疫功能中发挥重要作用。扁桃体上皮的原位染色研究表明，单层上皮角蛋白 K8、K18 和 K19 主要表达于隐窝上皮细胞。体外培养扁桃体上皮细胞可形成异质性的鳞状上皮细胞群，其分化过程仍在持续进行；同时，其角蛋白表达模式与体内扁桃体上皮细胞的表达特征一致。
ScienCell Research Laboratories 的人扁桃体上皮细胞（HTEpiC）分离自人扁桃体组织。HTEpiC 以第 1 代（P1）冻存，并以冷冻状态运输。每瓶含有 >5 × 105 个细胞，体积为 1 ml。HTEpiC 采用针对细胞角蛋白-18（cytokeratin-18）和/或细胞角蛋白-19（cytokeratin-19）的特异性抗体进行免疫荧光鉴定。HTEpiC 经检测对 HIV-1、HBV、HCV、支原体、细菌、酵母菌和真菌均呈阴性。在 ScienCell Research Laboratories 提供的培养条件下，HTEpiC 可进一步扩增 15 个群体倍增（population doublings）。
推荐培养基
推荐使用扁桃体上皮细胞培养基（Tonsil Epithelial Cell Medium，TEpiCM，产品编号：2561）在体外培养 HTEpiC。
| 目录编号 | 2560 |
|---|---|
| 制造国家 | 美国 |
| 产品编码 | HTEpiC |
| 规格/数量 | 5 x 10^5 cells/vial |
| 产品用途 | 本产品仅供研究使用。未经批准，不得用于人体、动物或体外诊断程序。 |
| 储存 | 收到后请立即将细胞从干冰直接转移至液氮中，并在进行细胞培养实验前始终保存在液氮中。 |
| 运输 | 干冰。 |
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