人扁桃体上皮细胞裂解液

Human Tonsil Epithelial Cell Lysate (HTEpiC Lysate) is prepared from early passage Human Tonsil Epithelial Cells using a modified RIPA buffer (50 mM Tris-HCl pH 7.2, 150 mM NaCl, 1% NP-40, 1mM EDTA, 1 mM EGTA, 0.4 mM PMSF, 5 μg/ml aprotinin, 5 μg/ml leupeptin, 1 μg/ml pepstatin, 1 mM Na3VO4, 5 mM NaF). Lysate concentration is determined by Beckman Coulter DU 800 spectrophotometer. Lysate is diluted and stored in Storage Buffer (Tris-HCl pH 6.8, 5% glycerol, 1% SDS, 0.002% bromphenol blue, 5% β-mercaptoethanol). Lysate from ScienCell Research Laboratories is convenient and cost effective for researchers as it eliminates the need to acquire expensive tissues.

人扁桃体上皮细胞裂解液（HTEpiC Lysate）由早期传代的人扁桃体上皮细胞制备而成，采用改良型 RIPA 裂解缓冲液（50 mM Tris-HCl，pH 7.2；150 mM NaCl；1% NP-40；1 mM EDTA；1 mM EGTA；0.4 mM PMSF；5 μg/ml 抑肽酶（aprotinin）；5 μg/ml 亮抑酶肽（leupeptin）；1 μg/ml 胃蛋白酶抑制剂（pepstatin）；1 mM Na₃VO₄；5 mM NaF）进行制备。裂解液浓度采用 Beckman Coulter DU 800 分光光度计测定。裂解液经稀释后储存于储存缓冲液中（Tris-HCl，pH 6.8；5% 甘油；1% SDS；0.002% 溴酚蓝；5% β-巯基乙醇）。ScienCell Research Laboratories 提供的细胞裂解液使用方便且具有较高的性价比，可免去研究人员获取昂贵组织样本的需要。

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