人源胸腺成纤维细胞

Fibroblasts have been extensively used for a wide range of cellular and molecular studies as they are one of easiest types of cells to grow in culture. Their durability also makes them amenable to a variety of manipulations ranging from studies employing gene transfection to microinjection. In general, fibroblasts secrete a non-rigid extracellular matrix (ECM) which is rich in type I and/or type III collagen. There is evidence showing that fibroblasts in various organs are intrinsically different. A previous study demonstrated that thymic fibroblasts are derived from the embryonic neural crest, which first surrounds the thymus as mesenchyme and later migrates into the thymic epithelium to form a network of fibroblasts. Thymic fibroblasts and the associated ECM have been shown to be responsible for normal thymus morphogenesis and lymphopoiesis.

HTyF from ScienCell Research Laboratories are isolated from the human fetal atrium. HTyF are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. HTyF are characterized by immunofluorescence with antibody specific to fibronectin. HTyF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HTyF are guaranteed to further expand for 15 population doublings under the conditions provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Fibroblast Medium (FM, Cat. #2301) for culturing HTyF in vitro.

成纤维细胞因其易于进行体外培养，被广泛应用于各种细胞和分子生物学研究。其较强的耐受性也使其适用于多种实验操作，包括基因转染和显微注射等研究。通常情况下，成纤维细胞可分泌富含Ⅰ型和/或Ⅲ型胶原蛋白的非刚性细胞外基质（ECM）。研究表明，来源于不同器官的成纤维细胞在生物学特性上存在内在差异。已有研究发现，胸腺成纤维细胞来源于胚胎神经嵴，该细胞群首先以间充质形式包围胸腺，随后迁移进入胸腺上皮组织，并形成成纤维细胞网络。胸腺成纤维细胞及其相关细胞外基质已被证实在正常胸腺形态发生和淋巴细胞生成过程中发挥重要作用。

ScienCell Research Laboratories 提供的人源胸腺成纤维细胞（HTyF）分离自人源胎儿心房组织。HTyF 以第 1 代细胞进行冻存，并以冷冻状态运输。每瓶含有超过 5 x 10⁵ 个细胞，体积为 1 ml。HTyF 经采用针对纤连蛋白的特异性抗体进行免疫荧光鉴定。HTyF 经检测为 HIV-1、HBV、HCV、支原体、细菌、酵母菌和真菌阴性。根据 ScienCell Research Laboratories 提供的培养条件，HTyF 可保证进一步扩增 15 个群体倍增周期。

推荐培养基

建议使用成纤维细胞培养基（FM，产品编号 #2301）对 HTyF 进行体外培养。