Human Stem Cell Pluripotency Detection qPCR Kit

The pluripotency of human embryonic and induced pluripotent stem cells (ESCs/iPSCs) are maintained by certain essential transcription factors¹. ScienCell has created a convenient qPCR kit for the assessment of these key pluripotency factors to ensure the quality of your human ESCs/iPSCs. NANOG, SOX2 and POU5F1 qPCR primers included in the kit allow for the detection and quantification of ESCs/iPSCs pluripotency.

Note: all gene names follow their official symbols by the Human Genome Organization Gene Nomenclature Committee (HGNC).

Four qPCR controls are included in this kit to verify successful reverse transcription of messenger RNA (mRNA) to complementary DNA (cDNA), reveal the presence of genomic DNA (gDNA) contamination in cDNA samples, and detect qPCR inhibitor contamination. Good quality cDNA is a critical component for successful gene expression analysis.

Each primer set included in HSC-qPCR kit arrives lyophilized in a 2 mL vial. All primers are designed and tested under the same parameters: (i) an optimal annealing temperature of 65°C (with 2 mM Mg2+, and no DMSO); (ii) recognition of all known target gene transcript variants; and (iii) specific amplification of only one amplicon. Each primer set has been validated by qPCR by melt curve analysis and gel electrophoresis.