Human Sertoli Cells

Sertoli Cells (SerC) are essential for testicular development, spermatogenesis, and formation of the blood-testis barrier. SerC limit the passage of substances such as hormones and nutrients to the adluminal compartment of the seminiferous tubules. In addition to forming the blood-testis barrier, SerC also provide the main structural support for the seminiferous tubules and protect the germ cells from the immune system. Aberrant SerC proliferation can contribute to the development of male reproductive disorders such as testicular germ-cell cancer, cryptorchidism, hypospadias, and low sperm count. SerC proliferation is in part controlled by folliclestimulating hormone (FSH) and thyroid hormone (TH), where FSH drives proliferation and TH promotes a more quiescent state. Cultured Human SerC are a useful in vitro model to better understand testicular dysgenesis syndrome and to develop treatments for male reproductive disorders.

HSerC from ScienCell Research Laboratories are isolated from human testis. HSerC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. HSerC are characterized by immunofluorescence with antibody specific to GATA-4 and Sox-9. HSerC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HSerC are guaranteed to further expand for 15 population doublings under the conditions provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Sertoli Cell Medium (SerCM, Cat. #4521) for the culturing of HSerC in vitro.