人睾丸支持细胞
ScienCell Research Laboratories 的人睾丸支持细胞（HSerC）分离自人睾丸组织。HSerC 以第 1 代（P1）冻存，并以冷冻状态运输。每瓶含有 >5 × 105 个细胞，体积为 1 ml。
Sertoli Cells (SerC) are essential for testicular development, spermatogenesis, and formation of the blood-testis barrier. SerC limit the passage of substances such as hormones and nutrients to the adluminal compartment of the seminiferous tubules. In addition to forming the blood-testis barrier, SerC also provide the main structural support for the seminiferous tubules and protect the germ cells from the immune system. Aberrant SerC proliferation can contribute to the development of male reproductive disorders such as testicular germ-cell cancer, cryptorchidism, hypospadias, and low sperm count. SerC proliferation is in part controlled by folliclestimulating hormone (FSH) and thyroid hormone (TH), where FSH drives proliferation and TH promotes a more quiescent state. Cultured Human SerC are a useful in vitro model to better understand testicular dysgenesis syndrome and to develop treatments for male reproductive disorders.
HSerC from ScienCell Research Laboratories are isolated from human testis. HSerC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 105 cells in 1 ml volume. HSerC are characterized by immunofluorescence with antibody specific to GATA-4 and Sox-9. HSerC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HSerC are guaranteed to further expand for 15 population doublings under the conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Sertoli Cell Medium (SerCM, Cat. #4521) for the culturing of HSerC in vitro.
睾丸支持细胞（Sertoli 细胞，SerC）对于睾丸发育、精子发生以及血-睾屏障的形成至关重要。SerC 可限制激素、营养物质等物质进入曲细精管的近腔室。在形成血-睾屏障的同时，SerC 还为曲细精管提供主要的结构支持，并保护生殖细胞免受免疫系统攻击。SerC 异常增殖可能参与睾丸生殖细胞癌、隐睾、尿道下裂和少精症等男性生殖系统疾病的发生。SerC 的增殖部分受到促卵泡素（FSH）和甲状腺激素（TH）的调控，其中 FSH 促进细胞增殖，而 TH 则促使细胞进入相对静息状态。培养的人睾丸支持细胞可作为有价值的体外模型，用于深入研究睾丸发育不良综合征，并探索男性生殖系统疾病的治疗方法。
ScienCell Research Laboratories 的人睾丸支持细胞（HSerC）分离自人睾丸组织。HSerC 以第 1 代（P1）冻存，并以冷冻状态运输。每瓶含有 >5 × 105 个细胞，体积为 1 ml。HSerC 采用针对 GATA-4 和 Sox-9 的特异性抗体进行免疫荧光鉴定。HSerC 经检测对 HIV-1、HBV、HCV、支原体、细菌、酵母菌和真菌均呈阴性。在 ScienCell Research Laboratories 提供的培养条件下，HSerC 可进一步扩增 15 个群体倍增（population doublings）。
推荐培养基
推荐使用睾丸支持细胞培养基（Sertoli Cell Medium，SerCM，产品编号：4521）在体外培养 HSerC。
| 目录编号 | 4520 |
|---|---|
| 制造国家 | 美国 |
| 产品编码 | HSerC |
| 规格/数量 | 5 x 10^5 cells/vial |
| 产品用途 | 本产品仅供研究使用。未经批准，不得用于人体、动物或体外诊断程序。 |
| 储存 | 收到后，立即将细胞从干冰转移到液氮中，并在液氮中保存，直到需要进行实验。 |
| 运输 | 干冰。 |
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