人直肠微血管内皮细胞

The human rectum is located in the lower gastrointestinal tract and functions as a temporary storage site for fecal material. Endothelial cells in the rectum line the interior surface of the blood vessels and form the interface between intravascular and extravascular compartments. Endothelial cells limit the passage of cellular and soluble substances between the blood vessels and the tissues. Endothelial cells also play critical roles in angiogenesis, vasculogenesis, and the inflammatory response. Rectal microvascular endothelial cells (RecMEC) have been implicated as a major contributing factor to the progression of rectal cancer. Human RecMEC (HRecMEC) are a useful in vitro model to elucidate the mechanisms of angiogenesis and to develop treatments for rectal cancer.

HRecMEC from ScienCell Research Laboratories are isolated from human rectum. HRecMEC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. HRecMEC are characterized by immunofluorescence with antibodies specific to vWF/Factor VIII and/or CD31 (PECAM1). HRecMEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HRecMEC are guaranteed to further expand for 10 population doublings under the conditions provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Endothelial Cell Medium (ECM, Cat. #1001) for culturing HRecMEC in vitro.

人直肠位于下消化道，是排泄物的暂时储存部位。直肠内的血管内皮细胞衬覆于血管内表面，构成血管内与血管外组织之间的界面。内皮细胞能够限制细胞及可溶性物质在血管与组织之间的通透，并在血管生成、血管发生和炎症反应中发挥重要作用。直肠微血管内皮细胞（RecMEC）被认为是促进直肠癌进展的重要因素。人直肠微血管内皮细胞（HRecMEC）可作为有价值的体外模型，用于阐明血管生成机制及开发直肠癌治疗方法。

ScienCell Research Laboratories 提供的人直肠微血管内皮细胞（HRecMEC）分离自人直肠组织。HRecMEC 在第一代（P1）阶段进行冻存，并以冷冻形式运输。每瓶含有超过 5 × 10⁵ 个细胞，总体积为 1 ml。HRecMEC 通过使用针对 von Willebrand 因子（vWF）/凝血因子 VIII 和/或 CD31（PECAM1）的特异性抗体进行免疫荧光染色鉴定。HRecMEC 经检测不含 HIV-1、HBV、HCV、支原体、细菌、酵母菌及真菌。在 ScienCell Research Laboratories 提供的培养条件下，HRecMEC 可保证进一步扩增至少 10 次群体倍增。

推荐培养基

建议使用内皮细胞培养基（Endothelial Cell Medium，ECM，产品编号 #1001）对 HRecMEC 进行体外培养。

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