人肺成纤维细胞（成人）
人肺成纤维细胞（成人）（HPF-a）分离自人成人肺组织。HPF-a 以第 1 代（P1）冻存，并以冷冻状态运输。每瓶含有 >5 × 105 个细胞，体积为 1 ml。
The most abundant cell type in lung interstitium is fibroblasts. They resemble ordinary fibroblasts but have some distinguishing features, such as long branching processes and gap junctions. HPF main function is to produce type III collagen, elastin, and proteoglycans of the extracellular matrix of the alveolar septa. Pulmonary fibroblasts (PF) play an important role in the repair and remodeling processes following injury. The controlled accumulation of fibroblasts to sites of inflammation is crucial to effective tissue repair after injury. Either inadequate or excessive accumulation of fibroblasts could result in abnormal tissue function. For example, the excess proliferation of fibroblasts contributes to the adventitial thickening observed during the development of hypoxia-induced pulmonary hypertension.
HPF-a from ScienCell Research Laboratories are isolated from adult human lung tissue. HPF-a are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 105 cells in 1 ml volume. HPF-a are characterized by immunofluorescence with antibody specific to fibronectin. HPF-a are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HPF-a are guaranteed to further expand for 15 population doublings under the conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Fibroblast Medium (FM, Cat. #2301) for the culturing of HPF-a in vitro.
成纤维细胞是肺间质中最丰富的细胞类型。它们与普通成纤维细胞形态相似，但具有一些独特特征，例如细长的分支状突起和缝隙连接。人肺成纤维细胞（HPF）的主要功能是合成肺泡隔细胞外基质中的Ⅲ型胶原蛋白、弹性蛋白和蛋白聚糖。肺成纤维细胞（PF）在肺组织损伤后的修复和重塑过程中发挥重要作用。损伤发生后，成纤维细胞受调控地聚集于炎症部位，对于有效的组织修复至关重要。成纤维细胞聚集不足或过度聚集均可能导致组织功能异常。例如，成纤维细胞过度增殖会促进肺动脉外膜增厚，这是缺氧诱导的肺动脉高压发生发展过程中常见的病理变化。
ScienCell Research Laboratories 的人肺成纤维细胞（成人）（HPF-a）分离自人成人肺组织。HPF-a 以第 1 代（P1）冻存，并以冷冻状态运输。每瓶含有 >5 × 105 个细胞，体积为 1 ml。HPF-a 采用针对纤连蛋白（fibronectin）的特异性抗体进行免疫荧光鉴定。HPF-a 经检测对 HIV-1、HBV、HCV、支原体、细菌、酵母菌和真菌均呈阴性。在 ScienCell Research Laboratories 提供的培养条件下，HPF-a 可进一步扩增 15 个群体倍增（population doublings）。
推荐培养基
推荐使用成纤维细胞培养基（Fibroblast Medium，FM，产品编号：2301）在体外培养 HPF-a。
| 目录编号 | 3310 |
|---|---|
| 制造国家 | 美国 |
| 产品编码 | HPF-a |
| 规格/数量 | 5 x 10^5 cells/vial |
| 产品用途 | 本产品仅供研究使用。未经批准，不得用于人体、动物或体外诊断程序。 |
| 储存 | 收到后立即将细胞从干冰直接转移至液氮中保存，直到需要进行细胞培养实验时再取出使用。 |
| 运输 | 干冰。 |
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