Human Pulmonary Fibroblast-adult Lysate

Quantity:200 μg Concentration: 4 mg/ml Storage buffer: 1X reducing sample buffer (50 mM Tris-HCl pH 6.8, 5% glycerol, 1% SDS, 0.002% bromphenol blue, 5% beta-mercaptoethanol) Lysis buffer: Modified RIPA buffer (50 mM Tris-HCl pH 7.2, 150 mM NaCl, 1% NP-40, 1mM EDTA, 1 mM EGTA, 0.4 mM PMSF, 5 μg/ml aprotinin, 5 μg/ml leupeptin, 1 μg/ml pepstatin, 1 mM Na3VO4, 5 mM NaF) Quality control: Lysate quality was assured with a NuPAGE 4-12% Bis-Tris Gel stained with Coomassie brilliant blue.