人肺泡上皮细胞

Pulmonary alveolar epithelial cells (PAEpiC), comprised of alveolar type I and type II epithelial cells, line more than 99% of the internal surface area of the lung. Type I cells are large squamous cells whose thin cytoplasmic extensions cover >95% of the internal surface area. They contain aquaporins and exhibit the highest osmotic water permeability of any mammalian cell type. Type II cells, which cover 2-5% of the surface area, produce, secrete, and recycle pulmonary surfactant. Type II cells contain Na⁺-, K⁺-ATPase and amiloride-sensitive epithelial Na+ channels. The currently accepted hypothesis is that Type II cells maintain pulmonary fluid homeostasis by regulating active Na⁺ transport in the lungs, whereas Type I cells are "inert" cells that provide solely a barrier function, rather than having active functions. Recent studies indicate
that Type I cells are also important in regulating ion and fluid transport.

HPAEpiC from ScienCell Research Laboratories are isolated from human lung tissue. HPAEpiC are cryopreserved at passage one and delivered frozen. Each vial contains >1 x 10⁶ cells in 1 ml volume. HPAEpiC are characterized by immunofluorescence with antibodies specific to cytokeratin-18 and/or cytokeratin-19. HPAEpiC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HPAEpiC are guaranteed to further culture under the conditions provided by ScienCell Research Laboratories; however, HPAEpiC are not recommended for expanding or long-term cultures since the cells differentiate to become type I alveolar epithelial cells immediately after plating and type I alveolar epithelial cells do not proliferate in culture.

Recommended Medium

It is recommended to use Alveolar Epithelial Cell Medium (AEpiCM, Cat. #3201) for culturing HPAEpiC in vitro.

肺泡上皮细胞（Pulmonary Alveolar Epithelial Cells，PAEpiC）由Ⅰ型和Ⅱ型肺泡上皮细胞组成，覆盖肺部超过 99% 的内部表面积。Ⅰ型肺泡上皮细胞为大型鳞状细胞，其薄层细胞质突起覆盖超过 95% 的肺泡内表面，含有水通道蛋白（Aquaporins），其渗透性水通透能力在哺乳动物细胞中处于最高水平。Ⅱ型肺泡上皮细胞覆盖约 2%–5% 的肺泡表面积，负责肺表面活性物质的合成、分泌与再循环，并表达 Na⁺/K⁺-ATP 酶及对阿米洛利（Amiloride）敏感的上皮 Na⁺ 通道。目前普遍认为，Ⅱ型肺泡上皮细胞通过调控肺组织中的主动 Na⁺ 转运维持肺液体稳态，而Ⅰ型肺泡上皮细胞主要发挥屏障功能。近年来的研究表明，Ⅰ型肺泡上皮细胞同样参与离子和液体转运的调控。

ScienCell Research Laboratories 提供的人肺泡上皮细胞（HPAEpiC）分离自人肺组织。HPAEpiC 于第 1 代（P1）进行冻存，并以冻存形式提供。每瓶含有 >1 × 10⁶ 个细胞，悬浮于 1 ml 冻存液中。HPAEpiC 经细胞角蛋白 18（Cytokeratin-18）和/或细胞角蛋白 19（Cytokeratin-19）特异性抗体免疫荧光鉴定。经检测，HPAEpiC 不含 HIV-1、HBV、HCV、支原体、细菌、酵母及真菌污染。ScienCell 保证 HPAEpiC 在推荐培养条件下能够继续培养；但由于细胞接种后会迅速分化为Ⅰ型肺泡上皮细胞，而Ⅰ型肺泡上皮细胞在体外培养中不具备增殖能力，因此不建议对 HPAEpiC 进行扩增培养或长期培养。

推荐培养基

建议使用肺泡上皮细胞培养基（Alveolar Epithelial Cell Medium，AEpiCM，产品编号：3201）进行 in vitro HPAEpiC 培养。