人肺泡上皮细胞（成人）

Pulmonary alveolar epithelial cells (PAEpiC), comprised of alveolar type I and type II epithelial cells, line more than 99% of the internal surface area of the lung. Type I cells are large squamous cells whose thin cytoplasmic extensions cover >95% of the internal surface area. They contain aquaporins and exhibit the highest osmotic water permeability of any mammalian cell type. Type II cells, which cover 2-5% of the surface area, produce, secrete, and recycle pulmonary surfactant. Type II cells contain Na⁺-, K⁺-ATPase and amiloride-sensitive epithelial Na⁺ channels. The currently accepted hypothesis is that Type II cells maintain pulmonary fluid homeostasis by regulating active Na+ transport in the lungs, whereas Type I cells are "inert" cells that provide solely a barrier function, rather than having active functions. Recent studies indicate
that Type I cells are also important in regulating ion and fluid transport.

HPAEpiC-a from ScienCell Research Laboratories are isolated from human adult lung tissue. HPAEpiC-a are cryopreserved at P0 and delivered frozen. Each vial contains >1 x 10⁶ cells in 1 ml volume. HPAEpiC-a are characterized by immunofluorescence with antibodies specific to cytokeratin-18, and/or cytokeratin-19. HPAEpiC-a are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HPAEpiC-a are guaranteed to further culture under the conditions provided by ScienCell Research Laboratories; however, HPAEpiC-a are not recommended for expanding or long-term cultures since the cells would differentiate to become type I alveolar epithelial cells immediately after plating and type I alveolar epithelial cells do not proliferate in culture.

肺泡上皮细胞（PAEpiC）由Ⅰ型和Ⅱ型肺泡上皮细胞组成，覆盖肺内部表面积的 99% 以上。Ⅰ型肺泡上皮细胞为大型鳞状细胞，其细薄的细胞质突起覆盖肺内部表面积的 95% 以上。这类细胞含有水通道蛋白，其渗透性水通透性在哺乳动物细胞中最高。Ⅱ型肺泡上皮细胞覆盖肺表面积的 2%–5%，主要负责肺表面活性物质的合成、分泌和再循环。Ⅱ型细胞表达 Na⁺-K⁺-ATPase 和对阿米洛利敏感的上皮钠通道。目前普遍认为，Ⅱ型肺泡上皮细胞通过调节肺部 Na⁺ 的主动转运来维持肺部液体稳态，而Ⅰ型肺泡上皮细胞则主要作为屏障发挥作用，被认为不具有主动调节功能。近期研究表明，Ⅰ型肺泡上皮细胞同样参与离子和液体转运的调节。

ScienCell Research Laboratories 的人肺泡上皮细胞（成人）（HPAEpiC-a）分离自人成人肺组织。细胞以 P0 代次冻存，并以冷冻状态运输。每瓶含有 >1 × 10⁶ 个细胞，体积为 1 ml。HPAEpiC-a 采用针对细胞角蛋白-18（cytokeratin-18）和/或细胞角蛋白-19（cytokeratin-19）的特异性抗体进行免疫荧光鉴定。HPAEpiC-a 经检测对 HIV-1、HBV、HCV、支原体、细菌、酵母菌和真菌均呈阴性。按照 ScienCell Research Laboratories 提供的培养条件，HPAEpiC-a 可进一步进行培养；但不建议用于细胞扩增或长期培养，因为这些细胞接种后会立即分化为Ⅰ型肺泡上皮细胞，而Ⅰ型肺泡上皮细胞无法在体外培养条件下增殖。

Search for a Certificate of Analysis by Lot Number below:

No Publication available at this time.