人内脏脂肪前体细胞

Adipocytes play an important role in energy storage and metabolism. Adipocyte differentiation is a developmental process that is critical for metabolic homeostasis and nutrient signaling. It is controlled by complex actions involving gene expression and signal transduction. Preadipocytes are present throughout adult life in adipose tissue and can proliferate and differentiate into mature adipocytes, contributing to increased adipose tissue mass. In vitro studies indicate that various tissue-derived preadipocytes exhibit different lipid accumulations, adipogenic transcription factor expression, and TNFα-induced apoptosis. There is also a close relationship between adipocyte differentiation and many physiological and pathological processes including fat metabolism, obesity, diabetes, hyperlipidemia, and breast cancer.

HPA-v from ScienCell Research Laboratories are isolated from human visceral fat tissue. HPA-v are cryopreserved at passage one and delivered frozen. Each vial contains >1 x 10⁶ cells in 1 ml volume. HPA-v are characterized by immunofluorescence with antibodies specific to CD44 or CD90, and lipid staining after differentiation. HPA-v are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HPA-v are guaranteed to further expand for 5 population doublings under the conditions provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Preadipocyte Medium (PAM, Cat. #7211) for the culturing of HPA-v in vitro. Preadipocyte Differentiation Medium (PADM, Cat. #7221) can be used for in vitro differentiation of preadipocytes into mature adipocytes, and then followed by Adipocyte Medium (AdM, Cat. #7201), which maintains mature adipocytes after differentiation.

脂肪细胞在能量储存和代谢过程中发挥着重要作用。脂肪细胞分化是维持机体代谢稳态和营养信号调控的关键发育过程，其受基因表达和信号转导等复杂机制的共同调控。脂肪前体细胞广泛存在于成年脂肪组织中，能够持续增殖并分化为成熟脂肪细胞，从而促进脂肪组织质量的增加。体外研究表明，不同组织来源的脂肪前体细胞在脂质积累、脂肪生成相关转录因子表达以及 TNF-α 诱导的细胞凋亡等方面存在显著差异。此外，脂肪细胞分化与脂质代谢、肥胖、糖尿病、高脂血症及乳腺癌等多种生理和病理过程密切相关。

ScienCell Research Laboratories 提供的人内脏脂肪前体细胞（HPA-v）分离自人内脏脂肪组织。HPA-v 以第 1 代（P1）状态进行冻存，并以冷冻形式运输。每支冻存管含有 >1 × 10⁶ 个细胞，体积为 1 ml。HPA-v 经免疫荧光检测，表达 CD44 或 CD90 等特异性标志物，并可在诱导分化后进行脂质染色鉴定。经检测，HPA-v 不含 HIV-1、HBV、HCV、支原体、细菌、酵母及真菌。在 ScienCell Research Laboratories 推荐的培养条件下，保证 HPA-v 可继续扩增至少 5 个群体倍增。

推荐培养基

建议使用脂肪前体细胞培养基（Preadipocyte Medium，PAM，产品编号：7211）进行 HPA-v 的体外培养。脂肪前体细胞分化培养基（Preadipocyte Differentiation Medium，PADM，产品编号：7221）可用于诱导脂肪前体细胞体外分化为成熟脂肪细胞，随后建议使用脂肪细胞培养基（Adipocyte Medium，AdM，产品编号：7201）维持分化后的成熟脂肪细胞。