Preadipocyte Differentiation Medium

Preadipocyte Differentiation Medium (PADM), when used with Preadipocyte Differentiation Supplement (PAdDS. Cat #7232) and 25 mL of fetal bovine serum (FBS) is a complete medium designed for the differentiation of preadipocytes into mature adipocytes in vitro. It is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors, trace minerals and a low concentration of fetal bovine serum (5%). The medium is HEPES and bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO₂ /95% air. The medium is formulated (quantitatively and qualitatively) to provide an optimally balanced nutritional environment that selectively supports the differentiation of preadipocytes into mature adipocytes in vitro.