人源心包成纤维细胞

Pericardial Fibroblasts (PcF) are the major cell type of the pericardium. They produce collagens and help form the thin but strong pericardium to serve as a protective sac for the heart by providing structural support and lubrication, and preventing excessive dilation of the heart. Dysregulated activation and proliferation of PcF, collagen and fibrin deposition, chronic inflammation, and scarring have been implicated in constrictive pericarditis, where pericardium hardening eventually prevents the heart from expanding. Associations between fibroblast activation and immunomodulation make PcF cultures a useful tool for studying progressive sclerosing pericarditis.

HPcF from ScienCell Research Laboratories are isolated from the human heart. HPcF are cryopreserved at passage one and are delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. HPcF are characterized by immunofluorescence with antibody specific to fibronectin. HPcF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HPcF are guaranteed to further expand for 15 population doublings under the conditions provided by
ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Fibroblast Medium (FM, Cat. #2301) for culturing HPcF in vitro.

心包成纤维细胞（PcF）是心包的主要细胞类型。它们可产生胶原蛋白，并参与形成薄而坚韧的心包组织，通过提供结构支持和润滑作用、预防心脏过度扩张，从而作为保护心脏的囊状结构。PcF 的异常激活和增殖、胶原蛋白及纤维蛋白沉积、慢性炎症以及瘢痕形成均与缩窄性心包炎的发生相关。在该疾病中，心包逐渐硬化，最终限制心脏扩张。成纤维细胞激活与免疫调节之间的关联，使 PcF 培养物成为研究进行性硬化性心包炎的有用工具。

ScienCell Research Laboratories 提供的人源心包成纤维细胞（HPcF）分离自人源心脏组织。HPcF 以第 1 代细胞进行冻存，并以冷冻状态运输。每瓶含有超过 5 x 10⁵ 个细胞，体积为 1 ml。HPcF 经采用针对纤连蛋白的特异性抗体进行免疫荧光鉴定。HPcF 经检测为 HIV-1、HBV、HCV、支原体、细菌、酵母菌和真菌阴性。根据 ScienCell Research Laboratories 提供的培养条件，HPcF 可保证进一步扩增 15 个群体倍增周期。

推荐培养基

建议使用成纤维细胞培养基（FM，产品编号 #2301）对 HPcF 进行体外培养。

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