Human Pericardial Fibroblast cDNA

Human Pericardial Fibroblast PCR-ready first strand cDNA (HPcF cDNA) is prepared from RNA extracted from Human Pericardial Fibroblasts by a Qiagen RNeasy kit. 2 ug total RNA is then reverse-transcribed using an Applied Biosystems' High-Capacity cDNA Reverse Transcription kit. 1 ul cDNA is sufficient for one PCR reaction. cDNA from ScienCell Research Laboratories is convenient and cost effective for researchers as it eliminates the need to acquire expensive tissues.