胰腺星形胶质细胞 (HPaSteC) 是胰腺的主要成纤维细胞。HPaSteC 负责合成和降解促进组织修复的细胞外基质蛋白。它们位于胰腺腺泡细胞旁边以及小胰腺导管和血管周围。当 HPaSteC 被激活时，它们会呈现类似肌成纤维细胞的形态，并分泌过量的细胞外基质蛋白，这可能导致慢性胰腺炎和腺癌的硬化。最近的研究表明，HPaSteC 还可以作为祖细胞、免疫细胞以及外分泌胰腺分泌的中介。此外，HPaSteC 刺激胰腺癌的增殖，抑制凋亡，并增强血管生成。HPaSteC 是研究胰腺肿瘤形成的理想模型，并可用于开发治疗慢性胰腺炎和腺癌的新疗法。
来自 ScienCell Research Laboratories 的 HPaSteC 是从人类胰腺分离的。HPaSteC 在第一级传代时被冷冻保存并交付。每瓶含有超过 5 x 105 细胞，体积为 1 毫升。HPaSteC 通过免疫荧光染色标定，使用特异性抗体对 α-平滑肌肌动蛋白进行标记。HPaSteC 对 HIV-1、HBV、HCV、支原体、细菌、酵母和真菌呈阴性反应。HPaSteC 保证在 ScienCell Research Laboratories 提供的条件下进一步扩增 15 次人口倍增。
推荐培养基
建议使用星形胶质细胞培养基 (SteCM, Cat. #5301) 来培养 HPaSteC。
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