人胰腺微血管内皮细胞

The pancreas is an endocrine gland and digestive organ which secretes hormones and produces pancreatic juice to aid in digestion. Pancreatic endocrine cells, also known as pancreatic islets, produce vital hormones such as glucagon, insulin, amylin, and somatostatin. In order to support the islets, the pancreas is a highly vascularized organ where endothelial cells play a critical role. Pancreatic microvascular endothelial cells (PaMEC) not only function to transport oxygen and nutrients to the pancreas, but also affect beta cell function and proliferation, influence insulin gene expression during islet development, and produce various growth factors associated with vasoactivity. Additionally, PaMEC are involved in optimizing blood glucose sensing and regulation. Recent studies have shown that PaMEC are involved in the development and progression of pancreatic cancer. Human PaMEC (HPaMEC) are a useful in vitro model for studying islet biology, pancreatic cancer, transplantation, and regenerative medicine.

HPaMEC from ScienCell Research Laboratories are isolated from human pancreas. HPaMEC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. HPaMEC are characterized by immunofluorescence with antibodies specific to vWF/Factor VIII and CD31 (PECAM). HPaMEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HPaMEC are guaranteed to further expand for 10 population doublings under the conditions provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Endothelial Cell Medium (ECM, Cat. #1001) for culturing HPaMEC in vitro

胰腺是一种内分泌腺体及消化器官，能够分泌激素并产生胰液以促进消化。胰腺内分泌细胞（亦称胰岛）可产生多种重要激素，包括胰高血糖素、胰岛素、胰淀素及生长抑素等。为支持胰岛功能，胰腺具有高度血管化的结构，其中内皮细胞发挥着关键作用。胰腺微血管内皮细胞（PaMEC）不仅负责向胰腺组织输送氧气和营养物质，还会影响β细胞功能与增殖，调控胰岛发育过程中的胰岛素基因表达，并分泌多种与血管活性相关的生长因子。此外，PaMEC 还参与血糖感知与调节的优化过程。最新研究表明，PaMEC 参与胰腺癌的发生与进展。人胰腺微血管内皮细胞（HPaMEC）是研究胰岛生物学、胰腺癌、移植以及再生医学的重要体外模型。

ScienCell Research Laboratories 提供的 HPaMEC 分离自人胰腺组织。HPaMEC 在第一代传代后进行冷冻保存，并以冻存状态交付。每瓶含有超过 5 x 10⁵ 个细胞，体积为 1 ml。HPaMEC 通过针对 vWF/凝血因子 VIII 及 CD31（PECAM）的特异性抗体进行免疫荧光鉴定。经检测，细胞不含 HIV-1、HBV、HCV、支原体、细菌、酵母及真菌污染。在 ScienCell Research Laboratories 提供的培养条件下，HPaMEC 可稳定扩增至少 10 次群体倍增。

推荐培养基

推荐使用 Endothelial Cell Medium（ECM，货号 #1001）用于 HPaMEC 的体外培养。