人卵巢表面上皮细胞

The ovary is covered by a single layer of surface epithelial cells (OSE) where in vivo morphology varies from squamous to cuboidal to low columnar. OSE actively participate in cyclical ovulatory follicular rupture and the subsequent repair. OSE express a variety of peptide hormones, sex steroids, growth factors, and receptors. Many of these biological agents have been implicated in the development of epithelial ovarian carcinomas and sexually transmitted infections. Understanding aberrations in the corresponding cellular pathways is of great clinical importance as they provide useful information regarding OSE cell survival, apoptosis, transformation, and function during disease development and potential markers for disease treatment.

HOSEpiC from ScienCell Research Laboratories are isolated from human ovary. HOSEpiC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. HOSEpiC are characterized by immunofluorescence with antibodies specific to cytokeratin-18 and/or cytokeratin-19. HOSEpiC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HOSEpiC are guaranteed to further expand for 5 population doublings under the conditions provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Ovarian Epithelial Cell Medium (OEpiCM, Cat. #7311) for culturing HOSEpiC in vitro.

卵巢表面覆盖着一层单层卵巢表面上皮细胞（OSE），其体内形态可呈现为鳞状、立方状或低柱状等多种形式。OSE 在周期性排卵过程中发挥重要作用，参与卵泡破裂以及随后发生的组织修复过程。OSE 可表达多种肽类激素、性激素、生长因子及其受体。其中许多生物活性分子被认为与上皮性卵巢癌的发生发展以及性传播感染相关。深入了解相关细胞信号通路中的异常变化对于临床研究具有重要意义，有助于阐明疾病发生过程中 OSE 细胞的存活、凋亡、转化及功能变化，并为疾病治疗提供潜在的生物标志物。

ScienCell Research Laboratories 提供的人卵巢表面上皮细胞（HOSEpiC）分离自人卵巢组织。细胞在第一代（P1）阶段进行冻存，并以冷冻形式运输。每瓶含有超过 5 × 10⁵ 个细胞，总体积为 1 ml。HOSEpiC 经免疫荧光染色鉴定，使用细胞角蛋白-18（cytokeratin-18）和/或细胞角蛋白-19（cytokeratin-19）特异性抗体进行检测。HOSEpiC 经检测不含 HIV-1、HBV、HCV、支原体、细菌、酵母菌及真菌。在 ScienCell Research Laboratories 提供的培养条件下，HOSEpiC 可保证进一步扩增至少 5 次群体倍增。

推荐培养基

建议使用卵巢上皮细胞培养基（Ovarian Epithelial Cell Medium，OEpiCM，产品编号 #7311）对 HOSEpiC 进行体外培养。